Analysis of the neoplastic nature and biological potential of sporadic and nevoid basal cell carcinoma syndrome-associated keratocystic odontogenic tumor
Analysis of the neoplastic nature and biological potential of sporadic and nevoid basal cell carcinoma syndrome-associated keratocystic odontogenic tumor
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DOI：
10.1111/j.1600-0714.2007.00578.x
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发表时间：
2007-10-01
影响因子：
3.3
通讯作者：
Nagai, Noriyuki
中科院分区：
文献类型：
--
作者：
Katase, Naoki;Nagatsuka, Hitoshi;Nagai, Noriyuki
背景技术背景：牙源性角化囊肿性肿瘤（keratocysticodontogenictumor，KCOT）是一种良性的囊性肿瘤，可合并痣样基底细胞癌综合征（nevoidbasalcellcarcinoma syndrome，NBCCS），表现为多发性囊性病变。尽管其组织学特征温和，但KCOT具有局部破坏性。然而，KCOT的肿瘤性质尚未得到很好的证实。乙酰肝素酶是一种内切-D-葡萄糖醛酸酶，特异性地切割硫酸乙酰肝素（HS），其在肿瘤中的水平的增加促进侵袭，血管生成和transferation.METHODS：探讨KCOT的肿瘤特性，我们研究了乙酰肝素酶在KCOT的定位模式，侧重于散发性和NBCCS相关的KCOTs之间的差异，通过免疫组化和原位杂交。结果：所有牙源性囊肿均呈不同程度的乙酰肝素酶阳性反应。乙酰肝素酶基因的表达模式与蛋白表达模式相一致。有趣的是，强烈的基因和蛋白质的表达，观察到在KCOT与NBCCS相比，散发的和含牙cystein.CONCLUSIONS：结果表明，乙酰肝素酶的表达可能与肿瘤性质的KCOT，特别是在NBCCS相关的情况下。
BACKGROUND: Keratocystic odontogenic tumor (KCOT), also known as odontogenic keratocyst, is a benign cystic neoplasm, which may be associated with nevoid basal cell carcinoma syndrome (NBCCS) and if it does, will occur as multiple cystic lesions. KCOT is locally destructive despite its bland histological features. However, the neoplastic nature of KCOT is not well established. Heparanase is an endo-D-glucuronidase enzyme that specifically cleaves heparan sulfate (HS) and the increase of its level in tumors promotes invasion, angiogenesis, and metastasis.METHODS: To investigate the neoplastic character of KCOT, we studied the localization patterns of heparanase in KCOT, focusing on the differences between sporadic and NBCCS-associated KCOTs, by immunohistochemistry and in situ hybridization. To compare the expression pattern of these cysts with non-tumorous odontogenic developmental cyst, dentigerous cyst was included.RESULTS: All the odontogenic cysts showed positive immunoreaction for heparanase protein in various intensities. The expression pattern of heparanase gene corresponded to that of protein expression. Interestingly, intense gene and protein expressions were observed in KCOT associated with NBCCS compared with sporadic ones and dentigerous cyst.CONCLUSIONS: The results implied that heparanase expression may be correlated with the neoplastic properties of KCOT, particularly in NBCCS-associated cases.