PHENOTYPES OF LYMPHOCYTES INFILTRATING NONSMALL CELL LUNG-CANCER TISSUES AND ITS VARIATION WITH HISTOLOGICAL TYPES OF CANCER
PHENOTYPES OF LYMPHOCYTES INFILTRATING NONSMALL CELL LUNG-CANCER TISSUES AND ITS VARIATION WITH HISTOLOGICAL TYPES OF CANCER
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DOI：
10.1016/0169-5002(93)90305-h
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发表时间：
1993-10-01
期刊：
影响因子：
5.3
通讯作者：
NOMOTO, K
中科院分区：
文献类型：
--
作者：
YOSHINO, I;YANO, T;NOMOTO, K
我们通过流式细胞术研究了手术中获得的四种不同组织学非小细胞肺癌组织中浸润的淋巴细胞的表面标志物以及40例未接受过抗癌治疗的临床病例的相应外周血淋巴细胞（PBL）。在估计每个淋巴细胞亚群的平均比例时，配对Wilcoxon检验显示，与PBL相比，肿瘤浸润淋巴细胞（TIL）的CD3 + 细胞（P < 0.05）、CD8 + 细胞（P < 0.05）和CD20 + 细胞（P < 0.01）值显着较高，而CD16 + 细胞值较低（P < 0.05）。 Spearman 检验显示，TIL 中的表面标记值与相应的 PBL 中的表面标记值均无统计学相关性（r < 0.6，P > 0.05）。虽然总TIL的平均CD4/CD8比值与PBL的平均CD4/CD8比值无显着差异，但12例鳞癌患者的TIL几乎总是显示出比PBL低的CD4/CD8比值(P < 0.05)，而腺癌中TIL和PBL的CD4/CD8比值相似。这些结果表明肺癌的TIL代表了特异性的免疫反应，其特征取决于肺癌的组织学类型。
We investigated surface markers of lymphocytes infiltrating four different histological non-small cell lung cancer tissues obtained at surgery and the corresponding peripheral blood lymphocytes (PBL) of 40 clinical cases, who had undergone no prior anti-cancer treatment, by flowcytometry. In estimation of the mean proportion of each lymphocyte subset, a paired Wilcoxon test revealed that tumor-infiltrating lymphocytes (TIL) exhibited significantly higher values of CD3+ cells (P < 0.05), CD8+ cells (P < 0.05) and CD20+ cells (P < 0.01), and lower value of CD16+cells (P < 0.05), as compared with those in PBL. The Spearman test revealed that none of the values of surface markers in TIL statistically correlated with those in the corresponding PBL (r < 0.6, P > 0.05). Although there was no significant difference between the mean CD4/CD8 ratio of total TIL and that of PBL, TIL of 12 patients with squamous cell carcinoma almost always showed lower values of CD4/CD8 ratio than those in PBL (P < 0.05), while the CD4/CD8 ratio was similar between TIL and PBL in adenocarcinoma. These results suggest that TIL of lung cancers represent specific immunological responses, whose characteristics depend on the histological types of lung cancers.