Differential induction of brain heme oxygenase-1 and heat shock protein 70 mRNA in sepsis.
Differential induction of brain heme oxygenase-1 and heat shock protein 70 mRNA in sepsis.
复制标题
脓毒症中脑血红素加氧酶 1 和热休克蛋白 70 mRNA 的差异诱导。
DOI：
--
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
R. Akagi
中科院分区：
文献类型：
--
作者：
T. Fujiwara;T. Takahashi;T. Suzuki;A. Yamasaki;M. Hirakawa;R. Akagi
我们研究了热休克蛋白70（HSP 70）和血红素加氧酶-1（HO-1）的基因表达，血红素加氧酶-1（HO-1）是血红素催化剂中的限速酶，也被称为热休克蛋白32（HSP 32），在大鼠脑内使用细菌脂多糖（LPS）诱导的脓毒症模型。腹腔注射LPS（10 mg/kg）可引起大鼠体温升高、白色细胞（WBC）计数升高、血清白细胞介素6（IL-6）水平明显升高，表现出典型的脓毒症病理特征。在该模型中，HO-1 mRNA在LPS给药后6 h开始升高，并持续升高至30 h。而HSP 70 mRNA仅在LPS刺激后3 ~ 6 h增加，12 h完全恢复到对照组水平。HO-1 mRNA主要表达于皮质和延髓，HSP 70 mRNA主要表达于纹状体。因此，HO-1和HSP 70 mRNA水平显示出独特的时间进程和组织分布在大脑中，这表明这些热休克蛋白（HSP）的基因表达分别调节。
We examined gene expression of heat shock protein 70 (HSP70) and heme oxygenase-1 (HO-1), which is the rate limiting enzyme in heme catabolism and is also known as heat shock protein 32 (HSP32), in the rat brain using a sepsis model induced by bacterial lipopolysaccharide (LPS). Intraperitoneal injection of LPS (10 mg/kg) to rats caused the elevation of body temperature and white blood cell (WBC) counts as well as marked elevation of serum interleukin-6 (IL-6) level, showing the typical pathological characteristics of sepsis. In this model, HO-1 mRNA increased at 6 h after LPS administration and continued to rise until 30 h. In contrast, HSP70 mRNA increased only between 3 h and 6 h after LPS administration, returning completely to the control level by 12 h. HO-1 mRNA was expressed predominantly in the cortex and the medulla oblongata, while HSP70 mRNA was expressed mainly in the striatum. HO-1 and HSP70 mRNA levels thus showed distinctive time courses and tissue distribution in the brain, suggesting that gene expression of these heat shock proteins (HSPs) is separately regulated.