A Ligation-PCR Approach for Generating Gene Replacement Constructs in Magnaporthe grisea
A Ligation-PCR Approach for Generating Gene Replacement Constructs in Magnaporthe grisea
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DOI：
10.4148/1941-4765.1137
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发表时间：
2004-09
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影响因子：
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通讯作者：
Xinhua Zhao;C. Xue;Yangseon Kim;Jin-Rong Xu
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文献类型：
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作者：
Xinhua Zhao;C. Xue;Yangseon Kim;Jin-Rong Xu
生成基因替代结构的传统方法涉及几个序列特异性克隆步骤，并且耗时。建立了一种连接- pcr方法来有效地生成基因替代结构。构建了两个适用于这种连接- pcr方法的载体和另一个适用于提高敲除突变体筛选效率的载体。本作品采用知识共享署名-相同方式共享4.0许可协议。这篇常规论文可在真菌遗传学报告：http://newprairiepress.org/fgr/vol51/iss1/7
The conventional approach for generating gene replacement constructs involves several sequence-specific cloning steps and is time-consuming. A ligation-PCR approach was developed to efficiently generate gene replacement constructs. Two vectors useful for this ligation-PCR approach and another vector suitable for improving the efficiency of knockout mutant screens were constructed. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol51/iss1/7