Characterization of pro-opiomelanocortin cDNA from the Old World monkey, Macaca nemestrina.
Characterization of pro-opiomelanocortin cDNA from the Old World monkey, Macaca nemestrina.
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旧世界猴 Macaca nemestrina 的阿片黑皮质素原 cDNA 的表征。
DOI：
10.1089/dna.1988.7.627
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Watson,SJ
中科院分区：
文献类型：
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作者：
Patel,PD;Sherman,TG;Watson,SJ
通过测定猴阿片黑色素原(β)前体的c DNA序列，研究了猴POMC-内啡肽(BE)与人或大鼠BE在化学上的差异。从猕猴全脑垂体cDNA文库中克隆了POMC的全长cDNA。最长的开放阅读框架预测了一个264个残基的多肽，展示了POMC的基本结构，与人类的同源密切。猴的BE序列明显偏离了人类的序列，后者将His-27改变为Tyr-27，但在Gln-31转变为Glu-31之前，使其比大鼠或人的BE更疏水性，这与其在反相高效液相色谱上的分析一致。将猴子POMC前体与其他物种的POMC前体进行比较，突出了保守的结构域，推测反映了有待阐明的生理活动区域。
An observation from high-pressure liquid chromatography (HPLC) suggesting that monkey β-endorphin (BE) was chemically different from human or rat BE was investigated by determining the cDNA sequence for the monkey pro-opiomelanocortin (POMC) precursor. A full-length cDNA for POMC was isolated from aMacaca nemestrinawhole pituitary cDNA library. The longest open reading frame predicts a 264-residue polypeptide exhibiting the basic structure of POMC that is closely homologous to the human counterpart. The monkey BE sequence apparently diverged from the human sequence after the latter had made the His-27 to Tyr-27 change but prior to the Gln-31 to Glu-31 transition, leaving it more hydrophobic than rat or human BE, consistent with its chromatography on reverse-phase HPLC. Comparison of the monkey POMC precursor with those of other species highlights conserved domains, presumably reflecting regions of physiological activity that await elucidation.