Properties of hemolysin and protease produced by Aeromonas trota.
Properties of hemolysin and protease produced by Aeromonas trota.
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DOI：
10.1371/journal.pone.0091149
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Okamoto K
中科院分区：
文献类型：
--
作者：
Takahashi E;Ozaki H;Fujii Y;Kobayashi H;Yamanaka H;Arimoto S;Negishi T;Okamoto K
本文研究了气单胞菌产生的外毒素的性质。enteropelogenes），气单胞菌科（Aeromonadaceae）的产气种之一。19 A的9在含有红细胞的固体培养基上生长的Trota分离物显示溶血活性。然而，这些溶血菌株的培养上清的溶血活性却不高。trota显著低于A. sobria在液体培养基中培养时的溶血素含量，以及用抗A. sobria也很低。用活细菌细胞进行的小鼠肠袢试验表明，A. trota 701引起大量体液蓄积，产生的溶血素抗血清可抑制A. trota 701.这些结果表明A. trota 701具有溶血性，产生的溶血素是引起A. trota。A.先前显示Sobria以预制体（无活性形式）分泌，并且当培养物上清液中的羧基末端结构域被蛋白酶切割掉时被激活。由于在A. trota，分析了A. trota。19 A的15在含有脱脂乳的固体培养基上生长的Trota分离物显示出蛋白水解活性。我们发现，大多数A。trota分离物具有丝氨酸蛋白酶基因，但不具有金属蛋白酶基因。因此，我们确定了丝氨酸蛋白酶基因及其伴侣蛋白A的核苷酸序列。trota基因。结果表明，丝氨酸蛋白酶和伴侣蛋白的氨基酸序列与A. sobria的同源性分别为83.0%和75.8%。
We examined the properties of exotoxins produced by Aeromonas trota (A. enteropelogenes), one of the diarrheagenic species of Aeromonadaceae. Nine of 19 A. trota isolates that grew on solid media containing erythrocytes showed hemolytic activity. However, the hemolytic activities of the culture supernatants of these hemolytic strains of A. trota were markedly lower than those of A. sobria when cultured in liquid medium, and the amount of hemolysin detected by immunoblotting using antiserum against the hemolysin produced by A. sobria was also low. A mouse intestine loop assay using living bacterial cells showed that A. trota 701 caused the significant accumulation of fluid, and antiserum against the hemolysin produced suppressed the enterotoxic action of A. trota 701. These results indicated that A. trota 701 was diarrheagenic and the hemolysin produced was the causative agent of the enterotoxic activity of A. trota. The hemolysin in A. sobria was previously shown to be secreted in a preform (inactive form) and be activated when the carboxy-terminal domain was cleaved off by proteases in the culture supernatant. Since mature hemolysin was detected in the culture supernatants of A. trota, we analyzed the extracellular protease produced by A. trota. Fifteen of 19 A. trota isolates that grew on solid media containing skim milk showed proteolytic activity. We subsequently found that most A. trota isolates possessed the serine protease gene, but not the metalloprotease gene. Therefore, we determined the nucleotide sequence of the serine protease gene and its chaperone A. trota gene. The results obtained revealed that the deduced amino acid sequences of serine protease and the chaperone were homologous to those of A. sobria with identities of 83.0% and 75.8%, respectively.