Platelet-activating factor in anaphylaxis and phagocytosis. I. Release from human peripheral polymorphonuclears and monocytes during the stimulation by ionophore A23187 and phagocytosis but not from degranulating basophils.
Platelet-activating factor in anaphylaxis and phagocytosis. I. Release from human peripheral polymorphonuclears and monocytes during the stimulation by ionophore A23187 and phagocytosis but not from degranulating basophils.
复制标题
过敏反应和吞噬作用中的血小板激活因子。
作者：
M. Sánchez;F. Alonso;J. Egido
血小板活化因子（PAF）是一种过敏反应的介质，最初在嗜碱性细胞中发现，后来在小鼠和大鼠巨噬细胞中发现。本文的目的是确定人白细胞释放PAF的细胞来源，并确定吞噬作用是否比过敏反应更重要地刺激PAF释放。吞噬白细胞（单核细胞和pmn）释放PAF，其物理化学性质类似于兔在酶聚糖、补体包被的酶聚糖、免疫复合物、免疫球蛋白聚集体或钙离子载体A23187攻击时产生的过敏反应。嗜碱性细胞不能通过抗人IgE抗体释放PAF，但发现脱颗粒和组胺释放呈阳性。吞噬白细胞与细胞松弛素B或秋水仙碱预孵育产生PAF释放减少，而β -葡糖苷酶释放增加。羧基肽酶B的添加对PAF和β -葡萄糖醛酸酶的释放没有显著影响。这些数据表明，从人白细胞制备中获得的PAF来自单核细胞和多形核；人嗜碱性细胞不能通过过敏性刺激或中性粒细胞阳离子蛋白释放可测量的PAF；PAF的释放和溶酶体含量遵循不同的机制，因为它们具有不同的动力学，并被作用于细胞骨架的药物以相反的方式修饰。
Platelet-activating factor (PAF) is a mediator of a anaphylaxis found initially in basophils and later in mouse and rat macrophages. The purpose of this paper was to determine the cellular origin of PAF released from human leucocytes and to establish if phagocytosis is a more important stimulus for PAF release than anaphylactic reactions. Phagocytic leucocytes (monocytes and PMNs) released PAF, physicochemically analogous to the PAF obtained by anaphylactic reactions in rabbits when challenged with zymosan, zymosan coated with complement, immune complexes, immunoglobulin aggregates or calcium ionophore A23187. Basophils failed to release PAF by anti-human IgE antibody, although positive degranulation and histamine liberaton were found. Pre-incubation of phagocytosing leucocytes with cytochalasin B or colchicine produced a diminution of PAF release, whereas beta-glucuronidase liberation was increased. The addition of carboxypeptidase B did not significantly modify PAF or beta-glucuronidase release. These data indicate that PAF obtained from preparations of human leucocytes comes from monocytes and polymorphonuclears; human basophils do not liberate measurable quantities of PAF, either by anaphylactic stimulus or by neutrophil cationic proteins; liberation of PAF and lysosomal content follow different mechanisms as they have different kinetics and are modified in an opposite way by drugs acting on the cytoskeleton.