Repeated exercise in mice alters expression of IL-10 and TNF-α in intestinal lymphocytes
Repeated exercise in mice alters expression of IL-10 and TNF-α in intestinal lymphocytes
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DOI：
10.1016/j.bbi.2007.07.002
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发表时间：
2008-02-01
影响因子：
15.1
通讯作者：
Guan, J.
中科院分区：
文献类型：
--
作者：
Hoffman-Goetz, L.;Spagnuolo, P. A.;Guan, J.
肠道炎症的特征是黏膜损伤，这种损伤可能部分是由于促炎细胞因子和抗炎细胞因子失衡引起的。本研究的目的是描述反复剧烈运动对小鼠肠道淋巴细胞（IL）中细胞因子表达的影响。34只雌性C57BL/6小鼠被随机分为三组：在跑步机上进行三次重复运动，每次间隔24小时，然后立即处死，或在24小时后处死，以及一个久坐（不运动）的对照组。通过蛋白质印迹法测定肠道淋巴细胞中的促炎细胞因子肿瘤坏死因子 -α（TNF -α）和抗炎细胞因子白细胞介素 - 10（IL - 10）。与久坐对照组相比，立即处死组的IL - 10浓度增加了48%（p < 0.05）。与立即处死组相比，运动方案完成24小时后小鼠肠道淋巴细胞中的TNF -α水平显著降低（p < 0.05）。结果表明可能存在一种生理代偿机制，即肠道淋巴细胞会因运动诱导的应激而增加IL - 10的表达。（C）2007爱思唯尔公司。保留所有权利。
Intestinal inflammation is characterized by mucosal damage that may arise, in part, to imbalances in pro- and anti-inflammatory cytokines. The purpose of this study was to describe the effects of repeated bouts of strenuous exercise on cytokine expression in mouse intestinal lymphocytes (IL). Thirty-four female C57BL/6 mice were randomly assigned to three groups: three repeated bouts of treadmill running separated by 24 h followed by sacrifice immediately or after a 24 h period or a sedentary (no exercise) control. The pro-inflammatory cytokine, TNF-alpha, and the anti-inflammatory cytokine, IL-10, were measured in IL by Western blotting. IL-10 concentration increased by 48% (p < 0.05) in the immediate group compared to the sedentary control. TNF-alpha levels in mouse IL were significantly lower 24 h after completion of the exercise protocol compared to the immediate group (p < 0.05). The results suggest a possible physiological compensation in which intestinal lymphocytes increase the expression of IL-10 in response to exercise-induced stress. (C) 2007 Elsevier Inc. All rights reserved.