Biomimetic 3D-printed neurovascular phantoms for near-infrared fluorescence imaging
Biomimetic 3D-printed neurovascular phantoms for near-infrared fluorescence imaging
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DOI：
10.1364/boe.9.002810
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发表时间：
2018-06-01
影响因子：
3.4
通讯作者：
Pfefer, T. Joshua
中科院分区：
文献类型：
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作者：
Liu, Yi;Ghassemi, Pejhman;Pfefer, T. Joshua
新兴的三维(3D)打印技术使得能够制造具有光学真实感和形态复杂的组织模拟体模，用于开发和评估新型光学成像产品。在这项研究中，我们评估了打印具有专利通道的图像定义神经血管模体用于对比度增强近红外荧光(NIRF)成像的可能性。从临床磁共振成像(MRI)定义的解剖图被分割并处理成适合在矩形和曲面仿生模体中打印前脑血管网络的文件。确定了有效清洗复杂血管标本的方法。最后一组模体使用定制的NIRF系统进行成像，激发波长为785 nm，使用两种NIRF造影剂。除了展示3D打印在创建高度逼真的、患者特定的生物光子模体方面的强大潜力外，我们的工作还提供了实现这一目标的最佳方法的见解，并阐明了这种方法目前的局限性。(C)2018年OSA开放获取出版协议条款下的美国光学学会
Emerging three-dimensional (3D) printing technology enables the fabrication of optically realistic and morphologically complex tissue-simulating phantoms for the development and evaluation of novel optical imaging products. In this study, we assess the potential to print image-defined neurovascular phantoms with patent channels for contrast-enhanced near-infrared fluorescence (NIRF) imaging. An anatomical map defined from clinical magnetic resonance imaging (MRI) was segmented and processed into files suitable for printing a forebrain vessel network in rectangular and curved-surface biomimetic phantoms. Methods for effectively cleaning samples with complex vasculature were determined. A final set of phantoms were imaged with a custom NIRF system at 785 nm excitation using two NIRF contrast agents. In addition to demonstrating the strong potential of 3D printing for creating highly realistic, patient-specific biophotonic phantoms, our work provides insight into optimal methods for accomplishing this goal and elucidates current limitations of this approach. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement