Site-dependent cleavage of pBR322 DNA by restriction endonuclease HinfI.
Site-dependent cleavage of pBR322 DNA by restriction endonuclease HinfI.
复制标题
通过限制性内切酶 HinfI 对 pBR322 DNA 进行位点依赖性切割。
DOI：
10.1093/nar/11.12.4109
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发表时间：
1983
影响因子：
14.9
通讯作者：
Bauer,WR
中科院分区：
文献类型：
--
作者：
Armstrong,KA;Bauer,WR
Cleavage of pBR322 DNA I by the restriction endonucleaseHinfI is preferentially inhibited at specificHinfI cleavage sites. These sites in pBR322 DNA I have been identified and ordered with respect to the frequency with which they are cleaved. TheHinfI site most resistant to cleavage in pBR322 DNA I is unique in that runs of G-C base pairs are immediately adjacent on both sides. Two differently permuted linear (DNA III) species were produced by cleavage with two different restriction endonucleases,PstI andAval. Only one of these linear molecules, that produced byPstI, exhibits the same preferential cleavage pattern as DNA I. The second linear species, that arising fromAvaI digestion, shows pronounced relative inhibition of cleavage at theHinfI sites nearest the ends of the molecule (100 and 120 base pairs away, respectively). This result suggests that proximity to the termini of a linear DNA molecule might also influence preferential cleavage. The possibility of formation of stem-loop structures does not appear to influence preferential cleavage byHinfI.
