Identification of a novel bluetongue virus vector species of Culicoides in Sicily
Identification of a novel bluetongue virus vector species of Culicoides in Sicily
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DOI：
10.1136/vr.153.3.71
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发表时间：
2003-07-19
影响因子：
2.2
通讯作者：
Mellor, PS
中科院分区：
文献类型：
--
作者：
Caracappa, S;Torina, A;Mellor, PS
蓝舌病病毒的媒介是某些种类的库蠓，在地中海地区，拟库蠓长期以来被认为是唯一的现场媒介。在西西里，自2000年秋季以来一直在实施一项昆虫学和血清学监测方案，该方案表明，C imicola的流行率和丰度低于意大利许多其他地区。此外，在2002年，有爆发的蓝舌病在没有C imicola，并在这些地区的蓝舌病毒RNA检测通过巢式逆转录酶PCR在野生捕获的，非充血，经产的库蠓pulicaris。此外，蓝舌病病毒血清型2的分离，在五个场合，从非充血的经产鸡Pulicaris提取物使用含胚鸡蛋和SHK-21细胞作为检测系统。这些发现表明，在意大利部分地区以及可能在欧洲其他地区，模仿念珠菌不存在或很少见，灰口念珠菌可能是蓝舌病毒的完全有效载体。
The vectors of bluetongue virus are certain species of Culicoides biting midges, and in the Mediterranean area Culicoides imicola has long been considered to be the only field vector. in Sicily an entomological and serological surveillance programme has been in operation since the autumn of 2000, which has shown that the prevalence and abundance of C imicola is lower than in many other Italian regions. Moreover, in 2002, there were outbreaks of bluetongue in the absence of C imicola, and in these regions bluetongue viral RNA was detected by means of a nested reverse-transcriptase PCR in wild-caught, non-blood-engorged, parous Culicoides pulicaris. Furthermore, bluetongue virus serotype 2 was isolated on five occasions from extracts of non-blood-engorged parous C pulicaris by using-embryonated hens eggs and SHK-21 cells as assay systems. These findings suggest that in parts of Italy and possibly in other areas of Europe, where C imicola is absent or rare, C pulicaris may act as a fully competent vector of bluetongue virus.