New Monoclonal Antibody B7 Selectively Recognizes Rat Myocardium Microvascular Endothelial Cells
New Monoclonal Antibody B7 Selectively Recognizes Rat Myocardium Microvascular Endothelial Cells
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新型单克隆抗体B7选择性识别大鼠心肌微血管内皮细胞
DOI：
10.1089/hyb.2010.0033
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发表时间：
2010-10-01
期刊：
影响因子：
--
通讯作者：
Fan, Daiming
中科院分区：
文献类型：
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作者：
Cui, Lina;Qiao, Lijuan;Fan, Daiming
心肌微血管内皮细胞(MMECs)在胚胎和成熟心肌的发育、存活和收缩过程中起着重要的调节作用。然而，MMECs的具体分子组成尚不清楚，也没有专门的MMEC标志物来区分微血管内皮细胞和大血管内皮细胞。在本研究中，我们用MMECs膜蛋白免疫BALB/c小鼠。通过酶联免疫吸附试验和免疫组织化学筛选，获得了一种新的特异性识别MMECs的单抗。组织芯片免疫组织化学显示，单抗B7还选择性地识别微血管内皮细胞，而不识别其他组织中的大血管内皮细胞。免疫荧光和免疫电子显微镜分析表明，B7抗原为质膜分子。有趣的是，我们还发现糖尿病大鼠的B7抗原与正常大鼠相比显著降低。综上所述，单抗B7不仅为我们了解微血管内皮细胞的分子组成提供了一个新的生物标志物，而且提示B7抗原在微血管内皮细胞功能障碍中可能起重要作用。
Myocardial microvascular endothelial cells (MMECs) play a vital role in modulating cardiomyocyte development, survival, and contraction during embryonic cardiogenesis and mature myocardium. However, specific molecular composition of MMECs is still not well known, with no special MMEC marker to distinguish microvascular endothelial cells (ECs) from macrovascular ECs. In the present study, we immunized BALB/c mice with membrane proteins of MMECs. Through screening by enzyme linked immunosorbent assay and immunohistochemistry, a new monoclonal antibody that specifically recognizes MMECs was yielded. Immunohistochemistry of tissue arrays showed that MAb B7 also selectively recognized microvascular ECs but not macrovascular ECs in other tissues. Immunofluorescence and immuno-electron microscope assay indicated that B7 antigen was a plasma membrane molecule. Interestingly, we also found that B7 antigen was dramatically decreased in diabetic rat compared with that in normal rat. In conclusion, MAb B7 not only provides a new biomarker to help us understand the molecular composition of microvascular ECs, but also indicates that B7 antigen might play an important role in the dysfunction of microvascular ECs.