Nebulized Menthol Impairs Mucociliary Clearance via TRPM8 and MUC5AC/MUC5B in Primary Airway Epithelial Cells.
Nebulized Menthol Impairs Mucociliary Clearance via TRPM8 and MUC5AC/MUC5B in Primary Airway Epithelial Cells.
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DOI：
10.3390/ijms24021694
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发表时间：
2023-01-15
影响因子：
5.6
通讯作者：
Salathe, Matthias
中科院分区：
文献类型：
--
作者：
Baumlin, Nathalie;Silswal, Neerupma;Dennis, John S.;Niloy, Asef J.;Kim, Michael D.;Salathe, Matthias
香料增强了电子烟（e-cigs）的适口性，薄荷醇仍然是电子烟用户的热门选择。薄荷味仍然是美国FDA批准用于商业化的基于豆荚的电子烟的唯一口味之一。然而，在电子烟中使用的高浓度吸入薄荷醇的安全性仍不清楚。在这里，我们测试了薄荷醇对原代气道上皮细胞气液界面（ALI）培养物中粘液纤毛清除（MCC）参数的影响。用基底外侧薄荷醇（1 mM）处理的ALI培养物显示24 h后纤毛搏动频率（CBF）和气道表面液体（ASL）体积显著降低。雾化到ALI培养物表面上的甲孕酮类似地减少CBF并增加粘液浓度，导致粘液纤毛运输速率降低。雾化薄荷醇进一步增加粘蛋白5AC（MUC 5AC）的表达和炎性细胞因子IL 1B和TNFA的mRNA表达。薄荷醇激活TRPM 8，且薄荷醇对MCC和炎症的作用可被TRPM 8特异性拮抗剂阻断。这些数据进一步证明，电子烟中使用的薄荷醇浓度可能会对气道造成伤害。
Flavorings enhance the palatability of e-cigarettes (e-cigs), with menthol remaining a popular choice among e-cig users. Menthol flavor remains one of the only flavors approved by the United States FDA for use in commercially available, pod-based e-cigs. However, the safety of inhaled menthol at the high concentrations used in e-cigs remains unclear. Here, we tested the effects of menthol on parameters of mucociliary clearance (MCC) in air–liquid interface (ALI) cultures of primary airway epithelial cells. ALI cultures treated with basolateral menthol (1 mM) showed a significant decrease in ciliary beat frequency (CBF) and airway surface liquid (ASL) volumes after 24 h. Menthol nebulized onto the surface of ALI cultures similarly reduced CBF and increased mucus concentrations, resulting in decreased rates of mucociliary transport. Nebulized menthol further increased the expression of mucin 5AC (MUC5AC) and mRNA expression of the inflammatory cytokines IL1B and TNFA. Menthol activated TRPM8, and the effects of menthol on MCC and inflammation could be blocked by a specific TRPM8 antagonist. These data provide further evidence that menthol at the concentrations used in e-cigs could cause harm to the airways.
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影响因子：
8
作者：
Fischer, Anthony J.;Pino-Argumedo, Maria, I;Abou Alaiwa, Mahmoud H.
通讯作者：
Abou Alaiwa, Mahmoud H.
DOI：
10.1152/ajplung.00304.2018
发表时间：
2019-03-01
影响因子：
4.9
作者：
Clapp, Phillip W.;Lavrich, Katelyn S.;Jaspers, Ilona
通讯作者：
Jaspers, Ilona
DOI：
10.1056/nejmoa1701632
发表时间：
2017-09-07
期刊：
The New England journal of medicine
影响因子：
--
作者：
Kesimer M;Ford AA;Ceppe A;Radicioni G;Cao R;Davis CW;Doerschuk CM;Alexis NE;Anderson WH;Henderson AG;Barr RG;Bleecker ER;Christenson SA;Cooper CB;Han MK;Hansel NN;Hastie AT;Hoffman EA;Kanner RE;Martinez F;Paine R 3rd;Woodruff PG;O'Neal WK;Boucher RC
通讯作者：
Boucher RC
影响因子：
15.9
作者：
Kim, Michael D.;Bengtson, Charles D.;Yoshida, Makoto;Niloy, Asef J.;Dennis, John S.;Baumlin, Nathalie;Salathe, Matthias
通讯作者：
Salathe, Matthias
影响因子：
10
作者：
Garcia-Arcos I;Geraghty P;Baumlin N;Campos M;Dabo AJ;Jundi B;Cummins N;Eden E;Grosche A;Salathe M;Foronjy R
通讯作者：
Foronjy R