Reactivity of 13 in vitro expressed hepatitis B surface antigen variants in 7 commercial diagnostic assays
Reactivity of 13 in vitro expressed hepatitis B surface antigen variants in 7 commercial diagnostic assays
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DOI：
10.1053/he.2000.6407
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发表时间：
2000-05-01
期刊：
影响因子：
13.5
通讯作者：
Carman, WF
中科院分区：
文献类型：
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作者：
Ireland, JH;O'Donnell, B;Carman, WF
目前乙型肝炎感染的主要标志物是表面抗原（HBsAg），但 HBsAg 阴性并不能排除乙型肝炎病毒血症，HBsAg 变异可能是导致此类诊断失败的原因。这里克隆了 13 种不同的 HBsAg 变体，在哺乳动物表达系统中产生变体蛋白，并使用 7 种商业 HBsAg 诊断分析进行测试。在分析的 12 个变体中，6 个样品在大多数检测中显示出与阳性对照（包含标准 HBsAg 序列）相似的反应性，但 6 个样品在整个主要亲水区 (MHR) 中含有各种突变，显示出反应性降低。发现 1 个样品中氨基酸 (aa) 124 处半胱氨酸的丢失影响了表达系统中 HBsAg 的分泌和反应性。因此，并非所有测定都同样能够检测 HBsAg 变异体，这意味着，为了达到可接受的灵敏度水平，应扩展当前测定的抗体库。
The primary marker of current hepatitis B infection is the surface antigen (HBsAg), however HBsAg negativity does not exclude hepatitis B viremia, HBsAg variants can be responsible for such diagnostic failures. Here 13 different HBsAg variants were cloned, variant protein produced in a mammalian expression system, and tested using 7 commercial HBsAg diagnostic assays. Of 12 variants analyzed, 6 samples displayed similar reactivity to the positive control (containing standard HBsAg sequence) in most of the assays, but 6 samples, containing various mutations throughout the entire major hydrophilic region (MHR), showed reduced reactivity, It was found that the loss of cysteine at amino acid (aa) 124 in 1 sample affected the secretion as well as the reactivity of HBsAg in the expression system. Thus, not all assays are equally able to detect HBsAg variants, implying that, to attain an acceptable level of sensitivity, the antibody repertoire of the current assays should be extended.