The contribution of mast cells to the late-phase of allergic asthma in rats
The contribution of mast cells to the late-phase of allergic asthma in rats
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DOI：
10.1007/s00011-005-1346-9
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发表时间：
2005-05-01
影响因子：
6.7
通讯作者：
Maeyama, K
中科院分区：
文献类型：
--
作者：
Liu, S;Chihara, K;Maeyama, K
简介：肥大细胞被认为是引起哮喘即刻反应的主要原因，但其在哮喘晚期反应中的作用尚不清楚，目的：通过晚期活化证实肥大细胞在过敏性哮喘晚期反应中的作用。结果：在整个哮喘发作期，哮喘+/+组大鼠气道收缩明显增强，尤其在哮喘晚期，哮喘+/+组大鼠气道收缩明显增强，哮喘+/+组气道收缩明显增强。+/+组大鼠支气管肺泡灌洗液组胺在激发后30 min和8 h分别增加5.3倍和3.4倍，而Ws/Ws组大鼠则无明显变化。在+/+大鼠气管上皮层中的粘膜肥大细胞的数量显着增加了2.2倍，在8h后的challenge.Conclusions，由原位杂交证明：肥大细胞可能有助于哮喘反应的晚期通过持续的肥大细胞激活和粘膜肥大细胞数量增加在哮喘反应的晚期。
Introduction: Mast cells are thought to be the main cause of an immediate asthmatic response, but their contribution to the late-phase of asthma is unknown.Objective: To prove the contribution of preactivated mast cells to the late phase of allergic asthma by advanced activation.Methods: Mast cell function in the late-phase of asthma was studied. Rats (wild, +/+ and mast cell deficient, Ws/Ws) were challenged with OVA to investigate the relationship between the contraction of airways and the population of inflammatory cells in the trachea.Result: During the entire asthmatic period, the contraction of the airway after OVA challenge in +/+ rats was enhanced significantly compared to Ws/Ws rats, especially in the late phase. The bronchoalveolar lavage fluid histamine in +/+, but not Ws/Ws, rats increased 5.3-fold in 30 min and 3.4-fold in 8 h after challenge, significantly. The number of mucosal mast cells in the tracheal epithelial layer in +/+ rats increased significantly 2.2-fold over controls at 8 h after challenge, as demonstrated by in situ hybridization.Conclusions: Mast cells may contribute to the late phase of asthmatic response by continuous mast cell activation and the mucosal mast cell number increased in the late phase of asthmatic response.