Selective influence of host microbiota on cAMP-mediated ion transport in mouse colon
Selective influence of host microbiota on cAMP-mediated ion transport in mouse colon
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DOI：
10.1111/nmo.12328
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发表时间：
2014-06-01
影响因子：
3.5
通讯作者：
Hyland, N. P.
中科院分区：
文献类型：
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作者：
Lomasney, K. W.;Houston, A.;Hyland, N. P.
远端结肠中的微生物比身体的任何其他部分都多，并且这种微生物群具有影响肠神经发育，兴奋性和胃肠道功能的能力。无菌（GF）小鼠是一种有价值的工具，在询问微生物与宿主之间的通信。尽管微生物群与结肠粘膜-粘膜下层之间存在密切关系，但研究微生物群对促分泌素诱发反应的影响的研究很少。为此，我们研究了上皮和神经诱发的离子转运，以及两种生物体引起的反应，在结肠粘膜下层制备GF小鼠在Ussing室。基线电参数，短路电流和跨上皮电阻，GF和常规动物的组织之间是相当的。然而，值得注意的是GF结肠对毛喉素刺激的高反应性。相比之下，没有微生物群并不影响组织对氨甲酰甲胆碱的反应。此外，相对于常规组织，GF小鼠对钠通道激活剂藜芦碱和TRPV 1受体激动剂辣椒素的反应得以保留。类似地，短路电流响应两个良好的表征寄生生物体发生独立的与宿主微生物群的相互作用。这是第一次全面表征分泌运动反应在GF结肠。
More microbes are resident in the distal colon than any other part of the body, and this microbiota has the capacity to influence enteric nerve development, excitability, and gastrointestinal function. Germ-free (GF) mice are a valuable tool in interrogating the communication between microbiota and host. Despite the intimate relationship which exists between the microbiota and the colonic mucosa-submucosa, there is a paucity of studies examining the influence of the microbiota on secretogogue-evoked responses. To this end, we investigated both epithelial and neural-evoked ion transport, and the response elicited by two commensal organisms, in colonic mucosa-submucosa preparations from GF mice in Ussing chambers. Baseline electrical parameters, short-circuit current and transepithelial resistance, were comparable between tissues from GF and conventional animals. Noteworthy, however, was a hyper-responsiveness of GF colon to forskolin stimulation. In contrast, the absence of the microbiota did not influence the tissue response to bethanechol. Moreover, responses to the sodium-channel activator, veratridine, and the TRPV1 receptor agonist, capsaicin were preserved in GF mice relative to conventional tissues. Similarly, the short-circuit current response to two well-characterized commensal organisms occurred independent of an interaction with the host microbiota. This is the first comprehensive characterization of secretomotor responses in GF colon.