M2-polarized macrophages in keratocystic odontogenic tumor: relation to tumor angiogenesis.
M2-polarized macrophages in keratocystic odontogenic tumor: relation to tumor angiogenesis.
复制标题
角化囊性牙源性肿瘤中的 M2 极化巨噬细胞：与肿瘤血管生成的关系
DOI：
10.1038/srep15586
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发表时间：
2015-10-28
影响因子：
4.6
通讯作者：
Zhao YF
中科院分区：
文献类型：
--
作者：
Zhong WQ;Chen G;Zhang W;Xiong XP;Zhao Y;Liu B;Zhao YF
本研究的目的是评估M2极化巨噬细胞的存在及其与角化囊性牙源性肿瘤（KCOT）血管生成的关系。通过免疫组织化学和免疫荧光检测KCOT样品中的M2极化巨噬细胞。同时，用抗CD 31抗体测量的微血管密度与M2极化巨噬细胞的存在密切相关。此外，巨噬细胞集落刺激因子（M-CSF）显着促进M2极化的巨噬细胞的激活。此外，体外伤口愈合、细胞迁移和管形成试验的结果进一步揭示了M2极化的巨噬细胞样细胞的促血管生成功能。这种功能可能与血管生成细胞因子如血管内皮生长因子（VEGF）、转化生长因子-β（TGF-β）和基质金属蛋白-9（MMP-9）的分泌有关。该研究首次证明M2极化巨噬细胞在KCOT中普遍存在，并且它们的存在取决于M-CSF表达。更重要的是，这些肿瘤支持细胞还可以通过分泌血管生成细胞因子促进肿瘤血管生成。
The purpose of this study was to evaluate the presence of M2-polarized macrophages and their relationships to angiogenesis in keratocystic odontogenic tumor (KCOT). M2-polarized macrophages were detected in KCOT samples by immunohistochemistry and immunofluorescence. Meanwhile, microvessel density measured with antibody against CD31 was closely correlated with the presence of M2-polarized macrophages. In addition, macrophage colony-stimulating factor (M-CSF) significantly contributed to the activation of M2-polarized macrophages. Moreover, the results ofin vitrowound healing, cell migration and tube formation assays further revealed the pro-angiogenic function of M2-polarized macrophage-like cells. This function might be associated with secretion of angiogenic cytokines, such as vascular endothelial growth factor (VEGF), transforming growth factor-β (TGF-β) and matrix metalloprotein-9 (MMP-9). This study demonstrates for the first time that M2-polarized macrophages are prevalent in KCOT and their presence is dependent on M-CSF expression. More importantly, these tumor-supportive cells can also promote tumor angiogenesis by secreting angiogenic cytokines.