A technique for stereotaxic recordings of neuronal activity in awake, head-restrained mice
A technique for stereotaxic recordings of neuronal activity in awake, head-restrained mice
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DOI：
10.1016/j.jneumeth.2008.11.014
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发表时间：
2009-03-30
影响因子：
3
通讯作者：
Heck, Detlef H.
中科院分区：
文献类型：
--
作者：
Bryant, Jeri L.;Roy, Snigdha;Heck, Detlef H.
传统上，对清醒动物行为过程中大脑活动的神经生理学记录是在灵长类动物中进行的，因为灵长类动物与人类的进化关系密切，行为技能也相当。然而，通过适当设计的行为任务，许多关于大脑如何控制行为的基本问题也可以在小型啮齿动物中得到解决。今天，小鼠神经遗传学的快速发展，包括人类大脑疾病小鼠模型的开发，为研究正常和病理性脑功能提供了独特和无与伦比的机会。在清醒和行为小鼠的神经元活动的记录实验程序的发展是一个重要的和必要的步骤，对正常和病理小鼠脑功能的神经生理学研究。在这里，我们描述了一种方法，立体定位记录的神经元活动，从头部抑制小鼠在流体舔。舔液体是啮齿类动物的一种自然和自发行为，小鼠在头部受限的条件下很容易进行。使用头部约束的准备允许在重复实验期间在多个部位记录隔离良好的单个单元。因此，可以测试大量神经元与行为的关系，并且可以生成行为相关神经元活动的详细空间图，如这里使用来自小脑中的舔相关浦肯野细胞的记录所例示的。(C)2008 Elsevier B.V.保留所有权利。
Neurophysiological recordings of brain activity during behavior in awake animals have traditionally been performed in primates because of their evolutionary close relationship to humans and comparable behavioral skills. However, with properly designed behavioral tasks, many fundamental questions about how the brain controls behavior can also be addressed in small rodents. Today, the rapid progress in mouse neurogenetics, including the development of mouse models of human brain disorders, provides unique and unparalleled opportunities for the investigation of normal and pathological brain function. The development of experimental procedures for the recording of neuronal activity in awake and behaving mice is an important and necessary step towards neurophysiological investigation of normal and pathological mouse brain function. Here we describe a method for stereotaxic recordings of neuronal activity from head-restrained mice during fluid licking. Fluid licking is a natural and spontaneous behavior in rodents, which mice readily perform under head-restrained conditions. Using a head-restrained preparation allows recordings of well-isolated single units at multiple sites during repeated experimental sessions. Thus, a large number of neurons can be tested for their relationship with behavior and detailed spatial maps of behavior related neuronal activity can be generated as exemplified here with recordings from lick-related Purkinje cells in the cerebellum. (C) 2008 Elsevier B.V. All rights reserved.