Headgroup conformation and lipid--cholesterol association in phosphatidylcholine vesicles: a 31P(1H) nuclear Overhauser effect study.
Headgroup conformation and lipid--cholesterol association in phosphatidylcholine vesicles: a 31P(1H) nuclear Overhauser effect study.
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磷脂酰胆碱囊泡中的头基构象和脂质-胆固醇关联：31P(1H) 核奥弗豪瑟效应研究。
DOI：
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发表时间：
1975
影响因子：
11.1
通讯作者：
R. Martin
中科院分区：
文献类型：
--
作者：
P. Yeagle;W. Hutton;C. Huang;R. Martin
在~（31）P的核磁共振谱中观察到了核Overhauser效应。核Overhauser效应的信息内容已被应用到磷脂酰胆碱囊泡的结构和动力学性质。在囊泡中，仅观察到理论最大核Overhauser效应增强的1/3。这一结果是由N-甲基质子和磷脂酰胆碱磷酸盐之间的偶极相互作用以及内部运动的相关时间为1.4 × 10（-9）秒来解释的。添加高达30%的胆固醇不改变核Overhauser效应增强或自旋晶格弛豫时间的囊泡。有人认为，胆固醇的OH基团是氢键的磷脂酰胆碱分子的酯羰基氧。
The nuclear Overhauser effect has been observed in the nuclear magnetic resonance spectra of 31P. The information content of the nuclear Overhauser effect has been applied to the structure and dynamic properties of phosphatidylcholine vesicles. In the vesicles only 1/3 of the theoretical maximum nuclear Overhauser effect enhancement is observed. This result is accounted for by dipolar interactions between the N-methyl protons and the phosphate of phosphatidylcholine, and a correlation time for internal motion of 1.4 X 10(-9) sec. Addition of up to 30% cholesterol does not change the nuclear Overhauser effect enhancement or spin-lattice relaxation time of the vesicles. It is argued that the OH group of cholesterol is hydrogen bonded to the ester carbonyl oxygen of the phosphatidylcholine molecules.