Rapid size distribution and purity analysis of gastric mucus glycoproteins by size exclusion chromatography multi-angle laser light scattering
Rapid size distribution and purity analysis of gastric mucus glycoproteins by size exclusion chromatography multi-angle laser light scattering
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DOI：
10.1016/0141-8130(95)01071-8
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发表时间：
1996-02-01
影响因子：
8.2
通讯作者：
Harding, SE
中科院分区：
文献类型：
--
作者：
Jumel, K;Fiebrig, I;Harding, SE
两种市售猪胃粘蛋白和两种新鲜粘蛋白制剂的重均摩尔质量和摩尔质量分布通过在线耦合多角度激光光散射的尺寸排阻色谱技术（SEC/MALLS）测定。两种商业样品均表现出比新制备的材料低得多的平均摩尔质量，并且含有更多杂质。另一方面：冷冻保存 18 个月后检查的新鲜粘蛋白制剂显示摩尔质量略有增加。
The weight average molar masses and molar mass distributions of two commercial pig gastric mucins and two fresh mucin preparations were determined by the technique of size exclusion chromatography coupled on-line to multi angle laser light scattering (SEC/MALLS). Both commercial samples exhibited much lower molar mass averages than the freshly prepared material and contained more impurities. On the other hand: a fresh mucin preparation examined after being stored frozen for 18 months revealed a slight increase in molar mass.