Investigating solution-phase protein structure and dynamics by hydrogen exchange mass spectrometry.
Investigating solution-phase protein structure and dynamics by hydrogen exchange mass spectrometry.
复制标题
DOI：
10.1002/0471140864.ps1706s58
复制
发表时间：
2009-11-01
影响因子：
--
通讯作者：
Engen, John R
中科院分区：
文献类型：
--
作者：
Morgan, Christopher R;Engen, John R
通过利用氢交换 (HX) 等标记方法，质谱可以揭示有关蛋白质构象、动力学和相互作用的许多细节。在本单元中，讨论了适用于 HX-MS 方案的氢交换理论，详细解释了 HX-MS 的实践（包括数据分析和解释），并重点介绍了大大增加从该技术获得的信息深度的最新技术进步。
By taking advantage of labeling methods such as hydrogen exchange (HX), many details about protein conformation, dynamics, and interactions can be revealed by mass spectrometry. In this unit, hydrogen exchange theory is discussed as it applies to HX-MS protocols, the practice of HX-MS including data analysis and interpretation is explained in detail, and recent advancements in technology which greatly increase the depth of information gained from the technique are highlighted.