Escherichia coli yjjPB genes encode a succinate transporter important for succinate production
Escherichia coli yjjPB genes encode a succinate transporter important for succinate production
复制标题
DOI：
10.1080/09168451.2017.1345612
复制
发表时间：
2017-01-01
影响因子：
1.6
通讯作者：
Abe, Keietsu
中科院分区：
文献类型：
--
作者：
Fukui, Keita;Nanatani, Kei;Abe, Keietsu
在厌氧条件下，大肠杆菌通过还原三羧酸循环从葡萄糖产生琥珀酸。然而，迄今为止，尚未在大肠杆菌中建立编码琥珀酸输出蛋白的基因。因此，我们尝试通过筛选大肠杆菌 MG1655 基因组文库来鉴定编码琥珀酸输出蛋白的基因。我们将 yjjPB 基因确定为编码琥珀酸转运蛋白的候选基因，该转运蛋白可增强泛菌在有氧条件下的琥珀酸产量。在谷氨酸棒杆菌菌株 AJ110655sucE1 中进行的互补测定表明，YjjP 和 YjjB 都是恢复琥珀酸生产所必需的。此外，在厌氧条件下，yjjPB 的缺失使大肠杆菌中的琥珀酸产量减少了 70%。综上所述，这些结果表明 YjjPB 构成了大肠杆菌中的琥珀酸转运蛋白，并且两个基因的产物都是琥珀酸输出所必需的。
Under anaerobic conditions, Escherichia coli produces succinate from glucose via the reductive tricarboxylic acid cycle. To date, however, no genes encoding succinate exporters have been established in E. coli. Therefore, we attempted to identify genes encoding succinate exporters by screening an E. coli MG1655 genome library. We identified the yjjPB genes as candidates encoding a succinate transporter, which enhanced succinate production in Pantoea ananatis under aerobic conditions. A complementation assay conducted in Corynebacterium glutamicum strain AJ110655sucE1 demonstrated that both YjjP and YjjB are required for the restoration of succinate production. Furthermore, deletion of yjjPB decreased succinate production in E. coli by 70% under anaerobic conditions. Taken together, these results suggest that YjjPB constitutes a succinate transporter in E. coli and that the products of both genes are required for succinate export.