In vitro transformation of chlorogenic acid by human gut microbiota
In vitro transformation of chlorogenic acid by human gut microbiota
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DOI：
10.1002/mnfr.201300441
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发表时间：
2014-05-01
影响因子：
5.2
通讯作者：
Rossi, Maddalena
中科院分区：
文献类型：
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作者：
Tomas-Barberan, Francisco;Garcia-Villalba, Rocio;Rossi, Maddalena
绿原酸（3- o -咖啡酰奎宁酸，C-QA）是奎宁酸的咖啡酯，是西方饮食中含量最多的酚酸之一。大部分C-QA在小肠中没有被吸收，到达结肠，在结肠中，常驻的微生物群将其转化为几种代谢物。比较了9名受试者肠道微生物群的C-QA转化，以评估细菌代谢的可变性。研究了一种能够水解C-QA的潜在益生菌双歧杆菌菌株是否会影响C-QA的命运。方法和结果利用不同被试菌群进行的生物转化实验表明，C-QA的代谢过程依次为氢化、脱氧和酯水解，在被试中发生的顺序不同。转化也可以通过奎宁酸残基分解进行，因为鉴定了咖啡因酰甘油中间体（HPLC-MS/MS, Q-TOF）。所有途径都汇聚在3-（3-羟基苯基）-丙酸上，在少数受试者中转化为羟基苯基乙醇和/或苯乙酸。将一株能水解C-QA的动物双歧杆菌添加到微生物群培养中。它影响微生物组成，但没有达到改变C-QA代谢的程度。结论提供了受试者之间微生物群C-QA转化的可变性图。首次描述了通过咖啡酰甘油中间体的转化途径。
ScopeChlorogenic acid (3-O-caffeoyl-quinic acid, C-QA), the caffeic ester of quinic acid, is one of the most abundant phenolic acids in Western diet. The majority of C-QA escapes absorption in the small intestine and reaches the colon, where the resident microbiota transforms it into several metabolites. C-QA conversion by the gut microbiota from nine subjects was compared to evaluate the variability of bacterial metabolism. It was investigated whether a potentially probiotic Bifidobacterium strain, capable of C-QA hydrolysis, could affect C-QA fate.Methods and resultsBioconversion experiments exploiting the microbiota from diverse subjects revealed that C-QA was metabolized through a succession of hydrogenation, dexydroxylation and ester hydrolysis, occurring in different order among the subjects. Transformation may proceed also through quinic acid residue breakdown, since caffeoyl-glycerol intermediates were identified (HPLC-MS/MS, Q-TOF). All the pathways converged on 3-(3-hydroxyphenyl)-propanoic acid, which was transformed to hydroxyphenyl-ethanol and/or phenylacetic acid in few subjects. A strain of Bifidobacterium animalis able to hydrolyze C-QA was added to microbiota cultures. It affected microbial composition but not to such an extent that C-QA metabolism was modified.ConclusionA picture of the variability of microbiota C-QA transformations among subjects is provided. The transformation route through caffeoyl-glycerol intermediates is described for the first time.