Hydrogen sulfide alleviates hypoxia-induced root tip death in Pisum sativum
Hydrogen sulfide alleviates hypoxia-induced root tip death in Pisum sativum
复制标题
硫化氢减轻豌豆缺氧引起的根尖死亡
DOI：
10.1016/j.plaphy.2013.05.042
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发表时间：
2013-09-01
影响因子：
6.5
通讯作者：
Wang, Chongying
中科院分区：
文献类型：
--
作者：
Cheng, Wei;Zhang, Liang;Wang, Chongying
土壤淹水通常会导致植物根部周围缺氧，这对作物来说是一种有害的非生物胁迫。硫化氢 (H2S) 是一种高度扩散的气态分子，可调节细胞信号传导并参与动物细胞的缺氧信号传导。然而，之前还没有关于植物细胞对缺氧反应的 H2S 的研究。通过分析内源 H2S 和活性氧 (ROS) 水平，研究了 H2S 对豌豆 (Pisum sativum) 缺氧诱导的根尖死亡的影响。使用分光光度测定法测定参与抗氧化和 H2S 代谢途径的关键酶的活性。通过气相色谱法测量乙烯。我们发现，外源 H2S 预处理可保护根尖细胞膜免受缺氧引起的 ROS 损伤，并通过抑制乙烯产生刺激静止策略，从而显着减轻缺氧引起的根尖死亡。相反，通过抑制负责内源 H2S 生物合成的关键酶，强烈增强缺氧引起的根尖死亡。我们的结果表明，外源 H2S 预处理可显着减轻豌豆幼苗缺氧引起的根尖死亡，从而增强植物对缺氧胁迫的耐受性。 (c) 2013 年 Elsevier Masson SAS。版权所有。
Flooding of soils often results in hypoxic conditions surrounding plant roots, which is a harmful abiotic stress to crops. Hydrogen sulfide (H2S) is a highly diffusible, gaseous molecule that modulates cell signaling and is involved in hypoxia signaling in animal cells. However, there have been no previous studies of H2S in plant cells in response to hypoxia. The effects of H2S on hypoxia-induced root tip death were studied in pea (Pisum sativum) via analysis of endogenous H2S and reactive oxygen species (ROS) levels. The activities of key enzymes involved in antioxidative and H2S metabolic pathways were determined using spectrophotometric assays. Ethylene was measured by gas chromatography. We found that exogenous H2S pretreatment dramatically alleviated hypoxia-induced root tip death by protecting root tip cell membranes from ROS damage induced by hypoxia and by stimulating a quiescence strategy through inhibiting ethylene production. Conversely, root tip death induced by hypoxia was strongly enhanced by inhibition of the key enzymes responsible for endogenous H2S biosynthesis. Our results demonstrated that exogenous H2S pretreatment significantly alleviates hypoxia-induced root tip death in pea seedlings and, therefore, enhances the tolerance of the plant to hypoxic stress. (c) 2013 Elsevier Masson SAS. All rights reserved.