Characterization of Dickeya strains isolated from potato and river water samples in Finland
Characterization of Dickeya strains isolated from potato and river water samples in Finland
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DOI：
10.1007/s10658-008-9274-5
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发表时间：
2008-10-01
影响因子：
1.8
通讯作者：
Pirhonen, Minna
中科院分区：
文献类型：
--
作者：
Laurila, Jaana;Ahola, Virpi;Pirhonen, Minna
从患病的马铃薯茎和块茎中分离到植物致病性肠杆菌Pectobacterium和Dickeya属（以前归类为Erwinia）。经PCR检测，分离得到的病原菌分别为atrosepticum、cartovorum和Dickeya属致病菌。此外，还从全国各地的河流水样中分离出Dickeya菌株。基于16S-23S rDNA基因间间隔序列的系统发育分析表明，Dickeya菌株可分为3个类群，其中2个从马铃薯中分离得到。用16S rDNA序列和在39℃下生长的系统发育分析表明，其中一个类群对应于D. dianthicola，这是一种温室栽培观赏植物的检疫病原体，而另外两个类群不明显类似于任何先前表征的Dickeya物种。田间试验结果表明，从河流样品中分离的dianthicola样菌株引起马铃薯茎部腐烂和坏死的发生率最高，但从马铃薯样品中分离的Dickeya菌株也引起症状。结果表明，尽管萎蔫杆菌仍是芬兰黑腿病的主要病因，但在马铃薯砧木和河流中普遍存在致病性迪克雅菌株。这是首次有报告表明Dickeya（一种热带和温暖气候中的病原体）可能导致北欧马铃薯疾病。
Plant pathogenic enterobacteria in the genera Pectobacterium and Dickeya (formerly classified as Erwinia) were isolated from diseased potato stems and tubers. The isolated bacteria were identified as P. atrosepticum, P. carotovorum and pathogens in the genus Dickeya with PCR tests. Furthermore, Dickeya strains were isolated from river water samples throughout the country. Phylogenetic analysis with 16S-23S rDNA intergenic spacer sequences suggested that the Dickeya strains could be divided into three groups, two of which were isolated from potato samples. Phylogenetic analysis with 16S rDNA sequences and growth at 39 degrees C suggested that one of the groups corresponds to D. dianthicola, a quarantine pathogen in greenhouse cultivation of ornamentals, while two of the groups did not clearly resemble any of the previously characterised Dickeya species. Field trials with the strains indicated that D. dianthicola-like strains isolated from river samples caused the highest incidence of rotting and necrosis of potato stems, but some of the Dickeya strains isolated from potato samples also caused symptoms. The results showed that although P. atrosepticum is still the major cause of blackleg in Finland, virulent Dickeya strains were commonly present in potato stocks and rivers. This is the first report suggesting that Dickeya, originally known as a pathogen in tropical and warm climates, may cause diseases in potato in northern Europe.