Low-abundance 32-kilodalton nuclear protein specifically enriched in the central nervous system.
Low-abundance 32-kilodalton nuclear protein specifically enriched in the central nervous system.
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低丰度 32 千道尔顿核蛋白，专门富集于中枢神经系统。
DOI：
10.1002/jnr.490250304
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发表时间：
1990
影响因子：
4.2
通讯作者：
Bigbee,JW
中科院分区：
文献类型：
--
作者：
Shelton,KR;Cunningham,JG;Klann,E;Merchant,RE;Egle,PM;Bigbee,JW
最近，在脑组织中发现了一种低丰度核蛋白 p32/6.3（Egle 和 Shelton：Journal of Biological Chemistry 261：2294–2298，1986）。使用蛋白质印迹程序，我们描述了它在神经系统中的分布，确定了它在大脑与肝脏、肾脏和某些其他组织中的相对富集，并描述了从大脑中的分离程序。选择性富集发生在基底神经节、间脑、海马、小脑、脑干、脊髓和大脑皮层，但不在视网膜、背根神经节和交感神经节中。因此，富集仅限于中枢神经系统区域。 p32/6.3 似乎优先在神经元中富集，因为在大鼠灰质的批量分离部分中，富含神经元的部分比富含星形胶质细胞的部分更丰富。来自前脑的不溶性核蛋白或基质组分中的 p32/6.3 浓度比来自肾脏、肝脏、肾上腺或视网膜的浓度高约 20 倍。这种丰富程度是一种古老的特征，在鸡和哺乳动物中都可以检测到。
Recently, a low‐abundance nuclear protein, p32/6.3, has been identified in brain tissue (Egle and Shelton: Journal of Biological Chemistry 261:2294–2298,1986). Using a Western blot procedure, we describe its distribution in the nervous system, determine its relative enrichment in brain versus liver, kidney, and certain other tissues, and describe an isolation procedure from brain. Selective enrichment occurs in basal ganglia, diencephalon, hippocampus, cerebellum, brainstem, spinal cord, and cerebral cortex but not in retina, dorsal root ganglia, and sympathetic ganglia. Thus, enrichment is limited to areas of the central nervous system. p32/6.3 appears to be preferentially enriched in neurons, because in bulk‐isolated fractions from rat grey matter it is more abundant in neuron‐enriched fractions than in astrocyte‐enriched fractions. p32/6.3 is approximately 20‐fold more concentrated in an insoluble nuclear protein or matrix fraction from forebrain than from kidney, liver, adrenal gland, or retina. This degree of enrichment is an ancient trait, detectable in the chicken as well as mammals.