Interaction of the E6 protein of human papillomavirus with cellular proteins.
Interaction of the E6 protein of human papillomavirus with cellular proteins.
复制标题
人乳头瘤病毒 E6 蛋白与细胞蛋白的相互作用。
作者：
N. Keen;R. Elston;L. Crawford
已知致癌人乳头瘤病毒（HPV）16型和18型的E6蛋白结合两种细胞蛋白，肿瘤抑制蛋白p53和称为E6-AP的100 kDa蛋白。在本文中，我们描述了生物活性E6融合蛋白的表达和纯化及其与其他细胞蛋白的特异性关联。HPV 16 E6与至少7种细胞蛋白特异性结合，根据分子量和磷酸化作用分别命名为pp 212、pp 182、p100、p81、p75、p53和p33。我们还表明，与HPV 16、18、6和11 E6蛋白相关的细胞蛋白复合物含有蛋白激酶。该蛋白激酶磷酸化外源性组蛋白H1和E6相关蛋白pp 182。
The E6 proteins of the oncogenic Human Papillomavirus (HPV) types 16 and 18 are known to bind two cellular proteins, the tumor suppressor protein p53 and a 100 kDa protein named E6-AP. In this paper we describe the expression and purification of biologically active E6 fusion proteins and their specific association with additional cellular proteins. HPV16E6 specifically associated with at least seven cellular proteins which have been designated pp212, pp182, p100, p81, p75, p53 and p33 respectively, on the basis of molecular mass and phosphorylation. We have also shown that the complex of cellular proteins associated with HPV16, 18, 6 and 11 E6 proteins contains a protein kinase. This protein kinase phosphorylated exogenous histone H1 and the E6 associated protein pp182.