Pseudomonas aeruginosa galU is required for a complete lipopolysaccharide core and repairs a secondary mutation in a PA103 (serogroup O11) wbpM mutant
Pseudomonas aeruginosa galU is required for a complete lipopolysaccharide core and repairs a secondary mutation in a PA103 (serogroup O11) wbpM mutant
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DOI：
10.1016/s0378-1097(02)00641-9
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发表时间：
2002-05-07
影响因子：
2.1
通讯作者：
Goldberg, JB
中科院分区：
文献类型：
--
作者：
Dean, CR;Goldberg, JB
铜绿假单胞菌血清群O 11菌株PA 103中wbpM的插入失活导致突变体表现出三种不同的脂多糖（LPS）表型。一个突变体PA 103 wbpM-C具有截短的LPS核心并且缺乏O抗原。这些缺陷没有被克隆的wbpM基因补充，表明存在二次突变。当将野生型galU基因导入含有克隆的wbpM基因的菌株PA 103 wbpM-C时，两种LPS缺陷都得到了纠正。在铜绿假单胞菌血清组O 11、O 5、O 6和O 17菌株中构建galU突变体导致LPS核心的截短，表明GalU参与铜绿假单胞菌LPS核心合成。(C)2002年由Elsevier Science B. V.代表欧洲微生物学会联合会出版。
Insertional inactivation of wbpM in Pseudomonas aeruginosa serogroup O11 strain PA103 resulted in mutants exhibiting three distinct lipopolysaccharide (LPS) phenotypes. One mutant, PA103 wbpM-C, had a truncated LPS core and lacked O antigen. These defects were not complemented by the cloned wbpM gene, suggesting a secondary mutation was present. When the wild-type galU gene was introduced into strain PA103 wbpM-C containing the cloned wbpM gene, both LPS defects were corrected. Construction of galU mutants in P. aeruginosa serogroups O11, O5, O6 and O17 strains led to truncation of the LPS core, indicating the involvement of GalU in P. aeruginosa LPS core synthesis. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.