Comparison of intestinal bacterial communities in grass carp, Ctenopharyngodon idellus, from two different habitats
Comparison of intestinal bacterial communities in grass carp, Ctenopharyngodon idellus, from two different habitats
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两种不同生境草鱼肠道细菌群落比较
DOI：
10.1007/s00343-012-1287-4
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发表时间：
2012-09-01
影响因子：
--
通讯作者：
Gao Lei
中科院分区：
文献类型：
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作者：
Ni Jiajia;Yu Yuhe;Gao Lei
脊椎动物的肠道细菌与宿主之间形成了密切的关系。宿主的外部和内部条件，包括其栖息地，都会影响肠道细菌群落。同样，肠道细菌群落可以反过来影响宿主，特别是在抗病方面。我们比较了从池塘和湖中采集的草鱼的肠道细菌群落。我们对扩增出的16S rRNA基因进行变性梯度凝胶电泳，从中鉴定出66个不同的分类单位。采用算术平均非加权对组法和主成分分析排序法发现，除DF-7外，两组池鱼的肠道细菌群落均聚在一起并嵌入到野生鱼类中，草鱼的遗传多样性与其肠道细菌群落之间没有显著的相关性(Mantel单尾检验，R=0.157，P=0.175)。在池塘采集的草鱼肠道中，盲肠杆菌的出现频率较高。
The intestinal bacteria of vertebrates form a close relationship with their host. External and internal conditions of the host, including its habitat, affect the intestinal bacterial community. Similarly, the intestinal bacterial community can, in turn, influence the host, particularly with respect to disease resistance. We compared the intestinal bacterial communities of grass carp that were collected from farm-ponds or a lake. We conducted denaturing gradient gel electrophoresis of amplified 16S rRNA genes, from which 66 different operational taxonomic units were identified. Using both the unweighted pair-group method with arithmetic means clustering and principal component analysis ordination, we found that the intestinal bacterial communities from the two groups of pond fish were clustered together and inset into the clusters of wild fish, except for DF-7, and there was no significant correlation between genetic diversity of grass carp and their intestinal bacterial communities (Mantel one-tailed test, R=0.157, P=0.175). Cetobacterium appeared more frequently in the intestine of grass carp collected from pond.