CD16+ monocyte subsets are increased in large abdominal aortic aneurysms and are differentially related with circulating and cell-associated biochemical and inflammatory biomarkers
CD16+ monocyte subsets are increased in large abdominal aortic aneurysms and are differentially related with circulating and cell-associated biochemical and inflammatory biomarkers
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DOI：
10.1155/2013/836849
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发表时间：
2013-01-01
期刊：
影响因子：
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通讯作者：
Palombo, Domenico
中科院分区：
文献类型：
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作者：
Ghigliotti, Giorgio;Barisione, Chiara;Palombo, Domenico
促炎成分存在于腹主动脉瘤 (AAA) 中。循环单核细胞表现出异质性，根据 CD14 和 CD16 受体的差异表达，已鉴定出三个亚群：CD14(+)CD16(-)、经典单核细胞、CD14(+)CD16(+)、中间单核细胞和 CD14(dim)CD16(+)、非经典单核细胞。 CKD 患者体内促炎性 CD16(+) 单核细胞增多且 CD143 高表达。 D-二聚体在 AAA 患者中增加，可能有助于与循环单核细胞相关的促炎反应。我们的目的是探讨AAA患者CD14(+)CD16(+)、CD14(dim)CD16(+)单核细胞和单核细胞CD143表达频率及其与D-二聚体、eGFR和其他炎症参数的关系。对74名AAA患者和30名健康对照者的血液进行分析，通过流式细胞术测定CD14(+)CD16(+)、CD14(dim)CD16(+)单核细胞和单核细胞CD143表达的频率。 AAA患者的CD16(+)亚群有所扩大（CD14(+)CD16(+)：7.66 +/- 0.31% vs 5.42 +/- 0.27%；CD14(dim)CD16(+)：7.43 +/- 0.48% vs 5.54 +/- 0.38%，AAA与对照组相比，平均值+/- SE，均p < 0.05）。 CD14(+)CD16(+)细胞与D-二聚体和年龄以及eGFR降低相关。 CD14(dim)CD16(+)细胞与尿酸、表面CD143以及白细胞总数和中性粒细胞计数减少有关。在 AAA 患者中，两个 CD16(+) 亚群和单核细胞 CD143 表达与 D-二聚体、肾功能参数以及循环生化和炎症生物标志物表现出不同的关系。
Proinflammatory components are present in abdominal aortic aneurysm (AAA). Circulating monocytes display heterogeneity, and three subsets have been identified, based on the differential expression for CD14 and CD16 receptors: CD14(+)CD16(-), classical, CD14(+)CD16(+), intermediate and CD14(dim)CD16(+), non-classical monocytes. Increased proinflammatory CD16(+) monocytes with high expression of CD143 are present in CKD patients. D-dimer is increased in AAA patients, and might contribute to the pro-inflammatory response associated to circulating monocytes. We aimed to investigate the frequency of CD14(+)CD16(+), CD14(dim)CD16(+) monocytes and monocyte CD143 expression in AAA patients, and their relationship with D-dimer, eGFR and other inflammatory parameters. Blood from 74 AAA patients and 30 healthy controls was analyzed to determine the frequency of CD14(+)CD16(+), CD14(dim)CD16(+) monocytes and the monocyte CD143 expression by means of flow-cytometry. AAA patients had expanded CD16(+) subsets (CD14(+)CD16(+): 7.66 +/- 0.31% vs 5.42 +/- 0.27%; CD14(dim)CD16(+): 7.43 +/- 0.48% vs 5.54 +/- 0.38%, AAA vs controls, mean +/- SE, both p < 0.05). CD14(+)CD16(+) cells were associated to D-dimer and age, and to reduced eGFR. CD14(dim)CD16(+) cells were associated to uric acid, surface CD143, and reduced count of total leukocytes and neutrophils. Within AAA patients, the two CD16(+) subsets and the monocyte CD143 expression display different relationships with D-dimer, parameters of renal function and circulating biochemical and inflammatory biomarkers.