Biobanking: The Future of Cell Preservation Strategies
Biobanking: The Future of Cell Preservation Strategies
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DOI：
10.1007/978-3-319-20579-3_4
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发表时间：
2015-01-01
期刊：
影响因子：
--
通讯作者：
Baust, John G.
中科院分区：
文献类型：
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作者：
Baust, John M.;Corwin, William L.;Baust, John G.
凭借成熟的技术，冷冻保存通常被视为“老派”学科，但现代冷冻保存正在经历另一个科学和技术发展的增长阶段。在这方面，当今的冷冻保存工艺和冷冻保存产品处于发现科学、干细胞研究、诊断开发和个性化医疗领域研究的前沿。随着冷冻细胞的利用率不断增加，对生物银行行业的要求也在不断增加并加速发展。提供解冻后立即高活力的样品不再足够。研究人员现在要求样品不仅具有高细胞回收率，而且回收的产品在基因组、蛋白质组、结构、功能和生殖水平上在生理和生化方面与其冷冻前状态相同。鉴于此，生物样本库现在面临着调整策略和协议以满足这些需求的挑战。最近的研究表明，细胞对低温保存的分子反应的控制和方向显着影响最终结果。本章概述了细胞对低温保存的分子应激反应、细胞凋亡和坏死细胞死亡连续体的影响，以及关注对冷冻温度的常见和/或细胞特异性反应的有针对性调节的研究如何提供提高样品质量和实用性的途径。这一研究方向提供了指导新研究、技术开发和程序的新方向和分子基础。随着冷冻保存的使用和知识库不断扩大，这条道路将继续改善整体功效和结果。
With established techniques cryopreservation is often viewed as an "old school" discipline yet modern cryopreservation is undergoing another scientific and technology development growth phase. In this regard, today's cryopreservation processes and cryopreserved products are found at the forefront of research in the areas of discovery science, stem cell research, diagnostic development and personalized medicine. As the utilization of cryopreserved cells continues to increase, the demands placed on the biobanking industry are increasing and evolving at an accelerated rate. No longer are samples providing for high immediate post-thaw viability adequate. Researchers are now requiring samples where not only is there high cell recovery but that the product recovered is physiologically and biochemically identical to its pre-freeze state at the genominic, proteomic, structural, functional and reproductive levels. Given this, biobanks are now facing the challenge of adapting strategies and protocols to address these needs moving forward. Recent studies have shown that the control and direction of the molecular response of cells to cryopreservation significantly impacts final outcome. This chapter provides an overview of the molecular stress responses of cells to cryopreservation, the impact of the apoptotic and necrotic cell death continuum and how studies focused on the targeted modulation of common and/or cell specific responses to freezing temperatures provide a path to improving sample quality and utility. This line of investigation has provided a new direction and molecular-based foundation guiding new research, technology development and procedures. As the use of and the knowledge base surrounding cryopreservation continues to expand, this path will continue to provide for improvements in overall efficacy and outcome.