Evidence and characterization of the receptor to eye-derived growth factor I, the retinal form of basic fibroblast growth factor, on bovine epithelial lens cells.
Evidence and characterization of the receptor to eye-derived growth factor I, the retinal form of basic fibroblast growth factor, on bovine epithelial lens cells.
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牛上皮晶状体细胞上眼源性生长因子 I（碱性成纤维细胞生长因子的视网膜形式）受体的证据和表征。
DOI：
10.1073/pnas.83.14.5024
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发表时间：
1986
影响因子：
11.1
通讯作者：
D. Barritault
中科院分区：
文献类型：
--
作者：
M. Moenner;B. Chevallier;J. Badet;D. Barritault
眼源性生长因子I（Eye-derived growth factor I，EDGF-I）是碱性成纤维细胞生长因子（basic fibroblast growth factor，bFGF）的视网膜形式。放射性碘标记的EDGF-I保留了完整的促有丝分裂活性，并用于研究生长因子与牛上皮透镜细胞的相互作用。我们发现，125I-标记的EDGF-I结合在一个饱和的和可逆的方式到一个特定的细胞受体。平衡结合的Scatchard分析得出Kd为53 × 10（-12）M，每个细胞约有20，000个结合位点。使用两种同型双功能试剂的交联实验诱导形成了一个特定的主要复合物，其Mr约等于145，000，由NaDodSO4/PAGE测定，并且与还原条件无关。这些数据证实了存在一种来自神经组织的碱性生长因子受体，并估计该受体的大小为Mr 130，000。
Eye-derived growth factor I (EDGF-I), the retinal form of the basic fibroblast growth factor, has been purified to homogeneity from bovine retina by heparin-Sepharose chromatography. The radioiodinated EDGF-I retained full mitogenic activity and was used to study the interaction of the growth factor with bovine epithelial lens cells. We showed that 125I-labeled EDGF-I bound in a saturable and reversible manner to a specific cellular receptor. Scatchard analysis of the equilibrium binding gave a Kd of 53 X 10(-12) M with approximately equal to 20,000 binding sites per cell. Crosslinking experiments using two homobifunctional reagents induced the formation of a specific major complex with a Mr of approximately equal to 145,000, as determined by NaDodSO4/PAGE, and independent of reducing conditions. These data establish the existence of a receptor for the basic growth factor derived from neural tissues and give an estimation of the size of this receptor at Mr 130,000.