Biologically important Thiols in various vegetables and fruits
Biologically important Thiols in various vegetables and fruits
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DOI：
10.1021/jf040266f
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发表时间：
2004-12-29
影响因子：
6.1
通讯作者：
Ercal, N
中科院分区：
文献类型：
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作者：
Demirkol, O;Adams, C;Ercal, N
生物硫醇是重要的抗氧化剂，最近的研究表明，它们的含量取决于食品的类别。因此，我们调查了一些生物硫醇在各种蔬菜和水果的水平，通过使用一个敏感的高效液相色谱（HPLC）技术。在一些蔬菜和水果中测量的生物硫醇包括谷胱甘肽（L-谷氨酰-L-半胱氨酸甘氨酸，GSH）、N-乙酰半胱氨酸（NAC）、卡托普利[CAP（C9 H15 NO 3S）]、高半胱氨酸（HCYS）、半胱氨酸（CYS）和γ-谷氨酰半胱氨酸（GGC）。我们的研究结果表明，生物硫醇含量在蔬菜中为3-349 nM/g湿重，在水果中为4-136 nM/g湿重。CAP仅在芦笋中发现（28 nM/g湿重）。此外，在卷心菜、红葡萄、黑莓、苹果和桃子中没有发现所分析的生物硫醇。因此，各种蔬菜和水果的巯基含量差异很大。这些重要的硫醇可能发生氧化，并导致有毒副产物的产生，如果它们暴露于辐射和臭氧处理用于灭菌目的。应进行进一步研究以监测这些生物硫醇的水平。
Biological thiols are important antioxidants, and recent studies showed that their contents vary depending on the groups of foodstuffs. Therefore, we investigated the levels of some biological thiols in various vegetables and fruits by using a sensitive high-performance liquid chromatography (HPLC) technique. Biological thiols measured in some vegetables and fruits include glutathione (L-glutamyl-L-cysteinly glycine, GSH), N-acetylcysteine (NAC), captopril [CAP (C9H15NO3S)], homocysteine (HCYS), cysteine (CYS), and gamma-glutamyl cysteine (GGC). Our results show that biological thiol contents are between 3-349 nM/g wet weight in vegetables and 4-136 nM/g wet weight in fruits. CAP is only found in asparagus (28 nM/g wet weight). Furthermore, none of the biological thiols analyzed were found in cabbages, red grapes, blackberries, apples, and peaches. Therefore, various vegetables and fruits differ significantly in their thiol contents. Oxidation of these important thiols may occur and result in the production of toxic byproducts, if they are exposed to radiation and ozone treatment for sterilization purposes. Further studies should be performed to monitor the levels of these biological thiols.