Fluid transfer in the everted human gallbladder.
Fluid transfer in the everted human gallbladder.
复制标题
外翻的人胆囊中的液体转移。
DOI：
10.1172/jci105562
复制
发表时间：
1967
期刊：
影响因子：
--
通讯作者：
J. A. Higgins
中科院分区：
文献类型：
--
作者：
G. Onstad;L. Schoenfield;J. A. Higgins
通过测量外翻制剂在受控条件下的重量变化来确定在胆石症手术中获得的人体胆囊液的转移率。液体从粘膜到浆膜表面的主动转运表明，由于相同的溶液在膜的两侧发生重量增加，并与流体静力学、渗透性和电势差异相反。关于钠，转移的液体与洗浴溶液是等渗的。新陈代谢抑制剂和极端温度会抑制体重增加。此外，这种体外制剂中的肌肉收缩与液体运动的速度和方向有关。在以前体重增加的制剂中，缩胆囊素增加了肌肉活动，并导致体重减轻。在所有被测试的制剂中，去甲肾上腺素导致体重增加或增加体重。外翻人体胆囊液的净运动方向是由主动粘膜运输的反作用力和肌肉收缩产生的过滤压力决定的。
The rates of fluid transfer across human gallbladders obtained at cholecystectomy for cholelithiasis were determined by the measurement of weight changes of everted preparations under controlled conditions. Active transport of fluid from the mucosal to the serosal surface was indicated since weight gain occurred with the same solution on both sides of the membrane and against hydrostatic, osmotic, and potential differences. With respect to sodium, the fluid transferred was isotonic to the bathing solutions. Metabolic inhibitors and temperature extremes inhibited weight gain. In addition, muscle contractions in this in vitro preparation were related to the rates and direction of fluid movement. Cholecystokinin increased muscle activity and caused weight loss in preparations that previously had gained weight. Norepinephrine caused weight gain or increased weight gain in all preparations tested. The direction of net fluid movement in the isolated everted human gallbladder was determined by the opposing forces of active mucosal transport and a filtration pressure generated by muscle contractions.