Measuring DNA modifications with the comet assay: a compendium of protocols.
Measuring DNA modifications with the comet assay: a compendium of protocols.
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使用彗星测定测量 DNA 修饰：协议概要。
DOI：
10.1038/s41596-022-00754-y
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发表时间：
2023-03
期刊：
影响因子：
14.8
通讯作者：
Azqueta, Amaya
中科院分区：
文献类型：
--
作者：
Collins, Andrew;Moller, Peter;Gajski, Goran;Vodenkova, Sona;Abdulwahed, Abdulhadi;Anderson, Diana;Bankoglu, Ezgi Eyluel;Bonassi, Stefano;Boutet-Robinet, Elisa;Brunborg, Gunnar;Chao, Christy;Cooke, Marcus S. S.;Costa, Carla;Costa, Solange;Dhawan, Alok;de Lapuente, Joaquin;Del Bo, Cristian;Dubus, Julien;Dusinska, Maria;Duthie, Susan J. J.;El Yamani, Naouale;Engelward, Bevin;Gaivao, Isabel;Giovannelli, Lisa;Godschalk, Roger;Guilherme, Sofia;Gutzkow, Kristine B. B.;Habas, Khaled;Hernandez, Alba;Herrero, Oscar;Isidori, Marina;Jha, Awadhesh N. N.;Knasmueller, Siegfried;Kooter, Ingeborg M. M.;Koppen, Gudrun;Kruszewski, Marcin;Ladeira, Carina;Laffon, Blanca;Larramendy, Marcelo;Le Hegarat, Ludovic;Lewies, Angelique;Lewinska, Anna;Liwszyc, Guillermo E. E.;de Cerain, Adela Lopez;Manjanatha, Mugimane;Marcos, Ricard;Milic, Mirta;de Andrade, Vanessa Moraes;Moretti, Massimo;Muruzabal, Damian;Novak, Matjaz;Oliveira, Rui;Olsen, Ann-Karin;Owiti, Norah;Pacheco, Mario;Pandey, Alok K. K.;Pfuhler, Stefan;Pourrut, Bertrand;Reisinger, Kerstin;Rojas, Emilio;Runden-Pran, Elise;Sanz-Serrano, Julen;Shaposhnikov, Sergey;Sipinen, Ville;Smeets, Karen;Stopper, Helga;Teixeira, Joao Paulo;Valdiglesias, Vanessa;Valverde, Mahara;van Acker, Frederique;van Schooten, Frederik-Jan;Vasquez, Marie;Wentzel, Johannes F. F.;Wnuk, Maciej;Wouters, Annelies;Zegura, Bojana;Zikmund, Tomas;Langie, Sabine A. S.;Azqueta, Amaya
彗星试验是一种检测单个真核细胞（从酵母到人类）核DNA损伤的通用方法。检测到的损伤类型包括DNA链断裂和碱不稳定位点（例如，脱嘌呤/脱嘧啶位点）、烷基化和氧化的核碱基、DNA-DNA交联、UV诱导的环丁烷嘧啶二聚体和一些化学诱导的DNA加合物。根据样本类型，彗星试验方案进行了重要修改，以避免在样本处理过程中形成额外的DNA损伤，并确保足够的灵敏度来检测样本组之间损伤水平的差异。彗星试验的各种应用已得到学术界、工业界和监管机构研究小组的验证，经济合作与发展组织采用彗星试验作为动物器官遗传毒性的体内试验，突出了彗星试验的优势。本文件包括一系列共识方案，其描述了彗星试验对各种细胞类型、物种和DNA损伤类型的应用，从而证明了其多功能性。
The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA–DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. Depending on the specimen type, there are important modifications to the comet assay protocol to avoid the formation of additional DNA damage during the processing of samples and to ensure sufficient sensitivity to detect differences in damage levels between sample groups. Various applications of the comet assay have been validated by research groups in academia, industry and regulatory agencies, and its strengths are highlighted by the adoption of the comet assay as an in vivo test for genotoxicity in animal organs by the Organisation for Economic Co-operation and Development. The present document includes a series of consensus protocols that describe the application of the comet assay to a wide variety of cell types, species and types of DNA damage, thereby demonstrating its versatility.
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影响因子：
2.5
作者：
Adamczyk J;Deregowska A;Panek A;Golec E;Lewinska A;Wnuk M
通讯作者：
Wnuk M
DOI：
10.1016/j.mrgentox.2011.05.010
发表时间：
2011-09-18
影响因子：
1.9
作者：
Azqueta, Amaya;Gutzkow, Kristine B.;Collins, Andrew R.
通讯作者：
Collins, Andrew R.
影响因子：
3.6
作者：
Akor-Dewu, Maryam B.;El Yamani, Naouale;Collins, Andrew R.
通讯作者：
Collins, Andrew R.
DOI：
10.1007/s00018-017-2697-4
发表时间：
2018-04
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Thiruvalluvan M;Barghouth PG;Tsur A;Broday L;Oviedo NJ
通讯作者：
Oviedo NJ
影响因子：
3.2
作者：
Azqueta, Amaya;Gutzkow, Kristine B.;Collins, Andrew R.
通讯作者：
Collins, Andrew R.