An Immunohistochemical and Polymerase Chain Reaction Evaluation of Feline Plasmacytic Pododermatitis
An Immunohistochemical and Polymerase Chain Reaction Evaluation of Feline Plasmacytic Pododermatitis
复制标题
猫浆细胞足皮炎的免疫组织化学和聚合酶链反应评估
DOI：
10.1354/vp.44-1-80
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发表时间：
2007
影响因子：
2.4
通讯作者：
Ralf S Mueller
中科院分区：
文献类型：
--
作者：
S. Bettenay;M. Lappin;Ralf S Mueller
对患有浆细胞性足皮炎的猫的 14 个皮肤活检切片以及 12-36 个月的临床随访进行了多克隆抗牛分枝杆菌（卡介苗 = BCG）抗体的染色，该抗体与广谱真菌和细菌有交叉反应。所有切片的实际足垫组织内的生物体均呈抗 BCG 抗体染色阴性。聚合酶链式反应 (PCR) 检测可扩增巴尔通体属、埃利希体属、嗜吞噬细胞无形体、猫衣原体、支原体属、刚地弓形体和猫疱疹病毒 1 (FHV-1) 的 DNA，并应用于组织消化物。所评估的那些病原体的 DNA 并未从组织中扩增。
Sections of 14 skin biopsies of cats with plasmacytic pododermatitis and a clinical follow-up of 12–36 months were stained with a polyclonal anti-Mycobacterium bovis (Bacille Calmette-Guerin = BCG) antibody cross-reactive to a broad spectrum of fungi and bacteria. All sections were negative for organisms within the actual footpad tissue with the anti-BCG antibody stains. Polymerase chain reaction (PCR) assays that amplify the DNA of Bartonella spp., Ehrlichia spp., Anaplasma phagocytophilum, Chlamydophila felis, Mycoplasma spp., Toxoplasma gondii, and feline herpesvirus 1 (FHV-1) were applied to tissue digests. DNA of those pathogens assessed was not amplified from tissue.