Androgen receptor in satellite cells is not essential for muscle regenerations
Androgen receptor in satellite cells is not essential for muscle regenerations
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卫星细胞中的雄激素受体对于肌肉再生并不是必需的
DOI：
10.1017/exp.2020.14
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发表时间：
2020
影响因子：
--
通讯作者：
Imai Y
中科院分区：
文献类型：
--
作者：
Sakai H;Sato T;Kanagawa M;Fukada S-i;Imai Y
雄激素对骨骼肌的合成代谢作用被认为是由雄激素受体（AR）介导的。虽然已经进行了多项关于AR在男性中作用的研究，但骨骼肌中AR的分子机制仍不清楚。在这里，我们首次证实了来自小鼠后肢肌肉的卫星细胞表达AR。然后，我们使用Pax 7 CreERT 2和ARL 2/Y小鼠产生卫星细胞特异性AR敲除小鼠，以测试卫星细胞中的AR是否是肌肉再生所必需的。令人惊讶的是，我们发现与ARL 2/Y小鼠相比，Pax 7 CreERT 2（Fan）/+对照小鼠和Pax 7 CreERT 2（Fan）/+; ARL 2/Y小鼠中的肌肉再生都受到损害。然而，Pax 7 CreERT 2（Gaka）/+; ARL 2/Y; R26 tdTomato/+小鼠在对照和突变体肌肉再生之间没有显示出显著差异。这些发现表明，卫星细胞中的AR对于肌肉再生不是必需的。我们建议Pax 7 CreERT 2（Fan）/+对照小鼠应包括在所有实验中，因为这些小鼠对肌肉再生有负面影响，并显示轻度再生表型。
The anabolic effects of androgen on skeletal muscles are thought to be mediated by androgen receptor (AR). Although multiple studies concerning the effects of AR in males have been performed, the molecular mechanisms of AR in skeletal muscles remain unclear. Here we first confirmed that satellite cells from mouse hindlimb muscles express AR. We then generated satellite cell-specific AR knockout mice using Pax7CreERT2 and ARL2/Y mice to test whether AR in satellite cells is necessary for muscle regeneration. Surprisingly, we found that muscle regeneration was compromised in both Pax7CreERT2(Fan)/+ control mice and Pax7CreERT2(Fan)/+;ARL2/Y mice compared to ARL2/Y mice. However, Pax7CreERT2(Gaka)/+;ARL2/Y;R26tdTomato/+ mice showed no significant differences between control and mutant muscle regeneration. These findings indicate that AR in satellite cells is not essential for muscle regeneration. We propose that Pax7CreERT2(Fan)/+ control mice should be included in all experiments, because these mice negatively affect the muscle regeneration and show the mild regeneration phenotype.