LEAD ASPARTATE, AN EN-BLOC CONTRAST STAIN PARTICULARLY USEFUL FOR ULTRASTRUCTURAL ENZYMOLOGY
LEAD ASPARTATE, AN EN-BLOC CONTRAST STAIN PARTICULARLY USEFUL FOR ULTRASTRUCTURAL ENZYMOLOGY
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DOI：
10.1177/27.10.512319
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发表时间：
1979-01-01
影响因子：
3.2
通讯作者：
WALTON, J
中科院分区：
文献类型：
--
作者：
WALTON, J
门冬氨酸铅是一种新的EM整体染色剂。其可预测的染色取决于螯合作用，该螯合作用是由硝酸铅和天冬氨酸这两种染色组分的相互作用产生的，这两种染色组分必须以特定的比例存在。天冬氨酸铅染色剂在硝酸铅中为0.02 M，在天冬氨酸中为0.03 M，调节至pH 5.5。细胞或组织在60 ℃下染色。天冬氨酸铅整体染色的细胞与柠檬酸铅在网格上染色的细胞非常相似，只是前者很少有污染。用天冬氨酸铅进行整体染色绕过了网格染色步骤，因此可以在薄切片后立即查看样品并拍照。天冬氨酸铅溶液的较低pH值允许溶解在高碱性柠檬酸铅染色剂中的酶反应产物的复染。门冬氨酸铅整体染色增强对比度，特别有利于需要干净和可预测的铅染色的超微结构研究。
Lead aspartate is a new en bloc stain for EM. Its predictable staining depends on chelation that results from the interaction of the 2 stain components, lead nitrate and aspartic acid, which must be present in a specific ratio. Lead aspartate stain is 0.02 M in lead nitrate and 0.03 M in aspartic acid, adjusted to pH 5.5. Cells or tissues are stained at 60.degree. C for 30-60 min. Cells stained en bloc with lead aspartate closely resemble cells stained on grids by lead citrate, except that the former seldom have contamination. En bloc staining with lead aspartate bypasses the grid-staining step so that samples can be viewed and photographed immediately after they are thin-sectioned. The lower pH of the lead aspartate solution allows counterstaining of enzyme reaction products that dissolve in the highly alkaline lead citrate stain. Lead aspartate en bloc staining to enhance contrast should especially benefit studies of ultrastructure requiring a clean and predictable lead stain.