Digital imaging scanning system and biomedical applications for biochips
Digital imaging scanning system and biomedical applications for biochips
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DOI：
10.1117/1.2939402
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发表时间：
2008-05-01
影响因子：
3.5
通讯作者：
Yang, Xiaoyong
中科院分区：
文献类型：
--
作者：
Huang, Guoliang;Deng, Cheng;Yang, Xiaoyong
生物芯片技术是世纪末发展起来的一种先进的生物医学技术。光学检测系统已成为生物芯片分析中非常重要的工具。微阵列芯片的高分辨率、大视场检测往往不能满足显微镜的要求，这就需要一些新的光学仪器。在这项工作中，一种新的数字成像扫描系统与暗场照射的微阵列芯片的一些生物医学应用，其特点是分析基因和蛋白质的临床样品与高特异性，平行，纳升的样品。该光学系统具有高数值孔径（NA = 0.72）、长工作距离（w（d）> 3.0mm）、良好的对比度和信噪比、接近3 μ m的高分辨力以及比其它商业共聚焦生物芯片扫描仪高两倍以上的荧光收集效率。提出了一种边缘重叠算法，用于自由区域检测的图像重构，并将扫描位置误差校正到1个像素的精度。为了方便地从扫描图像中识别目标，去除噪声，生成生物芯片分析的信号矩阵，探索了一种新的算法。数字成像扫描系统对于检测封闭的生物芯片与检测覆盖有盖玻片的载玻片表面上或培养液中的生物样品一样好。介绍了生物芯片在临床细菌鉴定和血清抗体检测中的应用。(C)2008年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。
Biochips have been an advanced technology for biomedical applications since the end of the 20th century. Optical detection systems have been a very important tool in biochip analysis. Microscopes are often inadequate for high resolution and big view-area detection of microarray chips, thus some new optical instruments are required. In this work, a novel digital imaging scanning system with dark-field irradiation is developed for some biomedical applications for microarray chips, characterized by analyzing genes and proteins of clinical samples with high specific, parallel, and nanoliter samples. The novel optical system has a high numerical aperture (NA = 0.72), a long working distance (w(d)>3.0 mm), an excellent contrast and signal-to-noise ratio, a high resolving power close to 3 mu m, and an efficiency of collected fluorescence more than two-fold better than that of other commercial confocal biochip scanners. An edge overlap algorithm is proposed for the image restructure of free area detection and correcting scanning position errors to a precision of 1 pixel. A novel algorithm is explored for recognizing the target from the scanning images conveniently, removing noise, and producing the signal matrix of biochip analysis., The digital imaging scanning system is equally as good for the detection of enclosed biochips as it is for the detection of biological samples on a slide surface covered with a glass cover slip or in culture solution. The clinical bacteria identification and serum antibody detection of biochips are described. (C) 2008 Society of Photo-Optical Instrumentation Engineers.