Increased association of CD38 with lipid rafts in T cells from patients with systemic lupus erythematosus and in activated normal T cells
Increased association of CD38 with lipid rafts in T cells from patients with systemic lupus erythematosus and in activated normal T cells
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DOI：
10.1016/j.molimm.2005.05.002
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发表时间：
2006-03-01
影响因子：
3.6
通讯作者：
Zubiaur, M
中科院分区：
文献类型：
--
作者：
Pavón, EJ;Muñoz, P;Zubiaur, M
在这项研究中，我们已经确定是否有CD38表达油T细胞，其分布在不同的膜微区，和T细胞活化SLE患者之间的关系。数据显示，在离体CD3（+）、CD4（+）、CD8（+）和CD25（+）SLE T细胞中，CD38表达增加，这与其在Brij 98去污剂中的不溶性增加以及其易位到脂筏中相关。此外，SLE T细胞显示出改变的CD4：CD8比率，这是由于CD4（+）T细胞的比例降低和伴随的CD8（+）T细胞的比例增加。这些数据是一致的增加CD38的表达和脂筏形成，并在有丝分裂原刺激的正常T细胞中观察到的CD4：CD8的比例显着降低相比，在离体未触及的正常T细胞。CD38在来自SLE T细胞或来自活化的正常T细胞的浮筏中的表达增加可以通过向接合的TCR提供或隔离信号传导分子来调节TCR信号传导。(C)2005爱思唯尔有限公司保留所有权利。
In this study we have determined whether there is a relationship between CD38 expression oil T cells, its distribution in different membrane microdomains, and T cell activation in SLE patients. The data show that CD38 expression is augmented in ex vivo CD3(+), CD4(+), CD8(+), and CD25(+) SLE T cells, which correlates with its increased insolubility in Brij 98 detergent, and its translocation into lipid rafts. Moreover, SLE T cells show an altered CD4:CD8 ratio, which is due to a decreased proportion of CD4(+) T cells and a concomitant increase in the proportion of CD8(+) T cells. These data are consistent with the increased CD38 expression and lipid raft formation, and the significant reduction in the CD4:CD8 ratio observed in mitogen-stimulated normal T cells as compared with that in ex vivo untouched normal T cells. Increased expression of CD38 in floating rafts from SLE T cells, or from activated normal T cells may modulate TCR signaling by providing or sequestering signaling molecules to the engaged TCR. (C) 2005 Elsevier Ltd. All rights reserved.