Chromosomal Control of Fertility in DROSOPHILA MELANOGASTER . I. Rescue of T(Y;A)/bb Male Sterility by Chromosome Rearrangement.
Chromosomal Control of Fertility in DROSOPHILA MELANOGASTER . I. Rescue of T(Y;A)/bb Male Sterility by Chromosome Rearrangement.
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果蝇生育力的染色体控制。
DOI：
10.1093/genetics/106.3.423
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发表时间：
1984
期刊：
影响因子：
3.3
通讯作者：
T. W. Lyttle
中科院分区：
文献类型：
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作者：
T. W. Lyttle
Y染色体和一个主要常染色体之间的许多易位对雄性黑腹果蝇的生育能力没有影响。然而，当这种易位携带者也携带着一条X染色体，而中心异染色质的很大一部分缺失时，他们通常是不育的。这被解释为是缺失和亚端帽的常染色体之间相互作用的结果。以此为起点，我们开发了一种辐射诱导易位重排的选择方案，该方案依赖于这样一个预测，即每当移位的常染色体顶端与常染色体着丝粒相关地被带回来时，在存在这样一个缺陷的X的情况下，生育能力就会恢复。这种观察和预测形成了所谓的男性生育常染色体连续性模型的基础。-这样的突变方案提供了几个优点。(1)它是有效的，在某些情况下产生1%以上的重封率。(2)方法简单；由于生育能力是选择性筛查的基础，许多男性可以在一个小瓶中进行测试。(3)它可用于同时产生原始易位断点附近的染色体材料所特有的重复和缺失。(4)突变的目标可以是成熟精子。-用该方法对从几个易位系获得的雄性可育重排模式的分析表明，在X短缩缺失的情况下，常染色体顶端及其着丝粒的连续性既不是男性生育的必要条件，也不是充分条件。因此，简单的常染色体连续性模型不足以解释染色体控制生育的这一复杂机制，必须进行相应的修订。文中还讨论了这一目标未来可能的研究方向。
Many translocations between the Y chromosome and a major autosome have no effect on the fertility of Drosophila melanogaster males. However, when such translocation-bearing males also carry an X chromosome deficient for a large portion of the centric heterochromatin, they are generally sterile. This has been interpreted to be the result of an interaction between the deficiency and the subterminally capped autosome. Using this observation as a starting point, we have developed a selection scheme for radiation-induced translocation resealings that depends on the prediction that fertility in the presence of such a deficient X is restored whenever the displaced autosomal tip is brought back in association with an autosomal centromere. The observation and the prediction form the basis for what is referred to as the autosomal continuity model for male fertility.-Such a mutagenesis scheme offers several advantages. (1) It is efficient, producing upward of 1% resealings in some cases. (2) It is simple; since fertility is the basis for the selective screen, many males can be tested in a single vial. (3) It can be used to simultaneously generate both duplications and deficiencies specific for chromosomal material adjacent to the original translocation breakpoints. (4) The target for mutagenesis can be mature sperm.-Analysis of the pattern of male-fertile rearrangements obtained from several translocation lines using this protocol indicates that continuity of the autosomal tips and their centromeres is neither a necessary nor sufficient condition for male fertility in the presence of a bobbed-deficient X. Thus, the simple autosomal continuity model is not adequate to explain this complicated mechanism of chromosomal control of fertility and will have to be revised accordingly. Potential future lines of inquiry toward this goal are discussed.