In vitro and in vivo detection of lactate with nanohybrid-functionalized Pt microelectrode facilitating assessment of tumor development
In vitro and in vivo detection of lactate with nanohybrid-functionalized Pt microelectrode facilitating assessment of tumor development
复制标题
使用纳米杂化功能化 Pt 微电极体外和体内检测乳酸有助于评估肿瘤发展
DOI：
10.1016/j.bios.2021.113474
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发表时间：
2021-07-08
影响因子：
12.6
通讯作者：
Wang, Lin
中科院分区：
文献类型：
--
作者：
Li, Qilin;Zhang, Yan;Wang, Lin
肿瘤细胞对葡萄糖的加速摄取和“有氧糖酵解”在细胞外空间和肿瘤组织内产生高水平的乳酸，这被认为是肿瘤的标志，并与肿瘤的发展密切相关。本文报道了一种基于Au纳米粒子修饰聚多巴胺纳米球（PDA-NS）的Pt微针电极的无酶电化学传感平台，并探讨了其在不同生物样品中乳酸的体外和体内检测中的实际应用。我们的研究结果表明，凭借纳米结构的优点和高的电催化活性，所得的纳米杂化微电极对乳酸的非酶电化学检测表现出良好的灵敏度和选择性，检测限为50 μ M，线性范围为0.375-12 mM，灵敏度为11.25 mA mM（-1）cm（-2），对其它活性小分子也有很好的抗干扰能力。该平台可以定量复杂生物液体中的乳酸盐，包括癌细胞和非癌细胞培养基以及血清样本，检测时间比临床使用的方法快7.5倍。此外，由于微型化的尺寸和令人满意的电化学性能，传感器实现了在活体肿瘤内与乳酸相关的特征伏安信号的体内记录，该信号与肿瘤负荷和生长正相关。因此，该平台不仅可用于癌症代谢研究，还可用于肿瘤进展的临床评估，甚至用于其他乳酸代谢紊乱的临床诊断。
Accelerated glucose uptake and "aerobic glycolysis" of tumor cells generates a high-level lactate in extracellular space and within tumor tissue, which is thought to be a hallmark of tumor and closely correlated with tumor development. Here, we report the development of an enzyme-free electrochemical sensing platform based on a Pt-microneedle electrode functionalized with Au nanoparticles (Au-NPs) decorated polydopamine nanospheres (PDA-NSs), and explore its practical application in in vitro and in vivo detection of lactate in different biological samples. Our results demonstrate that in virtue of the nanostructured merits and high electrocatalytic activity, the resultant nanohybrid-microelectrode exhibits good sensitivity and selectivity to the nonenzymatic electrochemical detection of lactate, with a detection limit of 50 mu M, a liner range of 0.375-12 mM, and a sensitivity of 11.25 mA mM(-1) cm(-2), as well as a good anti-interference ability to other active small molecules. The platform quantifies lactate in complex bio-fluids, including cancerous and non-cancerous cell culture media, as well as serum samples, with detecting time 7.5-fold faster than does a clinically-used approach. Moreover, owing to miniaturized size and satisfactory electrochemical performance, the sensor achieves in vivo recording of lactate-related characteristic voltammetric signals within a living tumor, which are positively correlated with tumor burden and growth. Therefore, the platform cannot only be employed for cancer metabolic investigation, but also potentially for clinical assessment of tumor progression, and even clinical diagnosis of other lactate metabolism disorders.