IMMUNODETECTION AND QUANTITATION OF THE 2 FORMS OF TRANSFORMING GROWTH FACTOR-BETA (TGF-BETA-1 AND TGF-BETA-2) SECRETED BY CELLS IN CULTURE
IMMUNODETECTION AND QUANTITATION OF THE 2 FORMS OF TRANSFORMING GROWTH FACTOR-BETA (TGF-BETA-1 AND TGF-BETA-2) SECRETED BY CELLS IN CULTURE
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DOI：
10.1002/jcp.1041380112
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发表时间：
1989-01-01
影响因子：
5.6
通讯作者：
SPORN, MB
中科院分区：
文献类型：
--
作者：
DANIELPOUR, D;DART, LL;SPORN, MB
转化生长因子β（TGF-β），在多种细胞类型中细胞生长、分化和细胞外基质成分表达的有效调节剂，作为两种不同的同源二聚体（TGF-β 1和RGF-β 2）存在，共有71%的序列同源性。放射受体和先前描述的使用兔抗体的放射免疫测定法不能区分这两种形式。我们已经在火鸡中开发了抗天然TGF-β 1和TGF-β 2的抗血清，它们各自特异性地阻断这些肽各自的受体结合和生物活性。使用这些免疫学试剂，我们描述了复杂生物流体中TGF-β 1和TGF-β 2的灵敏和特异性免疫学测定。使用这些测定，我们表明TGF-β 1和TGF-β 2都由多种培养细胞分泌，但是一些细胞主要分泌TGF-β 1或TGF-β 2，而另一些细胞以几乎相等的量分泌两种肽。我们的结果表明，两种形式的TGF-β的每一种的表达均与TGF-β受体的表达有关。是独立监管的。
Transforming growth factor beta (TGF-.beta.), a potent modulator of cell growth, differentiation, and the expression of extracellular matrix components in a variety of cell types, exists as two distinct homodimers (TGF-.beta.1 and RGF-.beta.2), sharing 71% sequence homology. Radioreceptor and previously described radioimmunological assays using rabbit antibodies have not been able to distinguish between these two forms. We have developed antisera in turkeys against native TGF-.beta.1 and TGF-.beta.2, each of which specifically blocks both the receptor binding and biological activity of each of these peptides. With these immunological reagents we describe sensitive and specific immunological assays for TGF-.beta.1 and TGF-.beta.2 in complex biological fluids. Using these assays we show that both TGF-.beta.1 and TGF-.beta.2 are secreted by a variety of cultured cells, but that some cells secrete predominantly either TGF-.beta.1 or TGF-.beta.2 while others secrete both peptides in nearly equal amounts. Our results demonstrate that the expression of each of the two forms of TGF-.beta. is independently regulated.