In vitro parasite-monocyte interactions in human leishmaniasis. Evidence for an active role of the parasite in attachment.
In vitro parasite-monocyte interactions in human leishmaniasis. Evidence for an active role of the parasite in attachment.
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人类利什曼病的体外寄生虫-单核细胞相互作用。
DOI：
10.1172/jci110606
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Wyler,DJ
中科院分区：
文献类型：
--
作者：
Wyler,DJ
利什曼原虫是哺乳动物宿主中单核吞噬细胞的专性细胞内寄生虫。为了更清楚地了解宿主-寄生虫相互作用的早期事件的特征，我们在体外将人单核细胞与热带利什曼原虫无鞭毛体进行了培养。单核细胞的感染是时间依赖的，并在4℃和细胞松弛素B存在的情况下受到抑制。用细胞松弛素预处理无鞭毛体可防止它们与正常单核细胞附着。未处理的无鞭毛体正常附着，但不能进入细胞松弛素预处理的单核细胞。这表明无鞭毛体积极参与依附，但内化需要宿主细胞的参与。
Leishmania are obligate intracellular parasites of mononuclear phagocytes in the mammalian host. To more clearly define features of the early events in host-parasite interaction, human monocytes were so-cultured with Leishmania tropica amastigotes in vitro. Infection of monocytes was time dependent and inhibited at 4 degrees C and in the presence of cytochalasin B. Pretreatment of amastigotes with cytochalasins prevented their attachment to normal monocytes. Untreated amastigotes attached normally but could not enter cytochalasin-pretreated monocytes. This suggests that amastigotes actively participate in attachment but require host cell participation for interiorization.Images