Combined effects of vitamin E and omega-3 fatty acids on protecting ambient PM2.5-induced cardiovascular injury in rats
Combined effects of vitamin E and omega-3 fatty acids on protecting ambient PM2.5-induced cardiovascular injury in rats
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DOI：
10.1016/j.chemosphere.2017.01.042
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发表时间：
2017-04-01
期刊：
影响因子：
8.8
通讯作者：
Zhao, Jinzhuo
中科院分区：
文献类型：
--
作者：
Du, Xihao;Jiang, Shuo;Zhao, Jinzhuo
目的：观察联合应用维生素E(VitE)和omega-3多不饱和脂肪酸(Omega-3 FA)能否通过减轻炎症反应和氧化应激来预防细颗粒物(PM2.5)所致的心血管损伤。同时，探索维生素E和Omega-3 FA的合适组合剂量，以寻找保护PM2.5损伤的最佳保护剂量。方法：用不同浓度的维生素E和Omega-3 FA单独或联合处理SD大鼠。然后，大鼠气管内滴注暴露于PM2.5环境中3次。采用酶联免疫吸附试验试剂盒检测血清和心肌组织上清液中肿瘤坏死因子-α(TNF-α)、白介素1β(IL-1β)、白介素6(IL-6)的表达。测定心肌丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)及血清丙二醛(MDA)水平。同时，通过组织病理学检查评价心脏损伤程度。结果：与PM2.5染毒组大鼠相比，维生素E或Omega-3 FA预处理组大鼠心脏损伤较轻。同时，Vit E或Omega-3 FA可显著降低炎性细胞因子(TNF-α、IL-1β、IL-6)，提高抗氧化活性，尤以联合应用Vit E和Omega-3 FA组更为明显。维生素E和Omega-3 FA的联合保护作用呈剂量依赖关系。结论：补充维生素E和Omega-3 FA对PM2.5诱导的损伤有保护作用，二者联合使用可能比单独添加更有效。(C)2017爱思唯尔有限公司。保留所有权利。
Objective: This study aims to observe whether the combined treatment with vitamin E (vit E) and omega-3 polyunsaturated fatty acids (Omega-3 FA) could prevent the fine particulate matter (PM2.5)-induced cardiovascular injury through alleviating inflammation and oxidative stress. At the same time, the appropriate combination dosage of vit E and Omega-3 FA was explored to find an optimized protective dose to protect the injury induced by PM2.5. Methods: The SD rats were pretreated with different concentration of vit E and Omega-3 FA separately or jointly. Then the rats were exposed to ambient PM2.5 by intratracheal instillation for three times. The expression of tumor necrosis factor a (TNF-alpha), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6) in serum and supernatant of cardiac tissue were detected by ELISA kits. The levels of malondialdehyde (MDA), superoxide Dismutase (SOD) and glutathione-peroxidase (GSH-Px) in myocardium and the level of MDA in serum were measured. Meanwhile, the cardiac injury was evaluated by histopathological examination. Results: Compared with the severe injury of rats in PM2.5 exposure group, the rats in vit E or Omega-3 FA-pretreated groups had a slighter injury in heart. Meanwhile, pretreatment with vit E or Omega-3 FA induced a significantly alleviation of the inflammatory cytokines (TNF-alpha, IL -1 beta, IL -6) and the elevation of the anti-oxidative activity especially in the rats pretreated with combined vit E and Omega-3 FA. In addition, the combined protecting effects of vit E and Omega-3 FA showed a dose dependent manner. Conclusion: Supplementation with vit E and Omega-3 FA could protect the PM2.5-induced injury, and the combination of vit E and Omega-3 FA might produce more effective effects than the separate nutrient did. (C) 2017 Elsevier Ltd. All rights reserved.