Cultured Sertoli cell-mediated FSH stimulatory effect on Leydig cell steroidogenesis.
Cultured Sertoli cell-mediated FSH stimulatory effect on Leydig cell steroidogenesis.
复制标题
培养的支持细胞介导的 FSH 对 Leydig 细胞类固醇生成的刺激作用。
作者：
M. Benahmed;J. Reventós;E. Tabone;J. Saez
为了确定支持细胞在卵泡刺激素(FSH)刺激间质细胞活性中的确切作用，将纯化的猪Leydigi细胞和Sertoli细胞分别或同时培养在不含或不含FSH(50 ng/mlFSH)的化学介质中，用两个参数评价间质细胞活性：人绒毛膜促性腺激素(HCG)结合部位；hCG刺激的cAMP生成和睾酮分泌。首先，研究发现，FSH可提高粗制间质细胞(40-60%的间质细胞)中间质细胞的活性，而对纯化的间质细胞(大于90%的间质细胞)无影响。第二，FSH可刺激与支持细胞共培养的间质细胞的活性，但对单独培养的纯化的间质细胞无明显影响。FSH对间质细胞活性的刺激作用依赖于共培养的Sertoli细胞数量。这些结果表明：1)FSH对间质细胞功能的刺激作用是由支持细胞介导的；2)支持支持细胞对间质细胞功能的局部调控。
To determine the precise role of Sertoli cells in the stimulating effects of follicle stimulating hormone (FSH) on Leydig cell activity, porcine purified Leydig and Sertoli cells were cultured separately or together in a chemically defined medium in the absence or presence of porcine, FSH 50 ng/ml. Leydig cell activity was evaluated using two parameters: human chorionic gonadotropin (hCG) binding sites; and hCG-stimulated cAMP production and testosterone secretion. First, it was found that FSH increases Leydig cell activity in crude Leydig cell preparations (40-60% of Leydig cells), whereas it exerts no effect on purified Leydig cells (greater than 90% of Leydig cells). Second, FSH stimulates the activity of Leydig cells cocultured with Sertoli cells, whereas it remains without effect on purified Leydig cells cultured alone. This stimulating effect of FSH on Leydig cell activity is dependent on the Sertoli cell number in the coculture. These data 1) show that the stimulating effect of FSH on Leydig cell function is mediated by Sertoli cells and 2) support the concept of local control of Leydig cell function originating from Sertoli cells.