Investigation of two-dimensional high performance liquid chromatography approaches for reversed phase resolution of warfarin and hydroxywarfarin isomers.
Investigation of two-dimensional high performance liquid chromatography approaches for reversed phase resolution of warfarin and hydroxywarfarin isomers.
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研究华法林和羟基华法林异构体反相解析的二维高效液相色谱方法。
DOI：
10.1016/j.chroma.2014.08.025
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
C. Welch
中科院分区：
文献类型：
--
作者：
E. Regalado;J. Schariter;C. Welch
研究了几种离线和在线2D HPLC方法，用于密切相关的华法林和羟基华法林异构体的复杂混合物的反相拆分。通过将反相非手性/手性HPLC分离与UV触发级分收集和随后对收集的级分进行手性/非手性反相HPLC分析相结合，可以完全分离混合物的所有12种组分。此外，开发了一种更快的方法，从在线二维HPLC分析，其中多组分馏分从第一维同时在第二维色谱。
Several offline and online 2D HPLC methods were investigated for the reversed phase resolution of a complex mixture of closely related warfarin and hydroxywarfarin isomers. By combining reversed phase achiral/chiral HPLC separation with UV-triggered fraction collection and subsequent chiral/achiral reversed phase HPLC analysis of collected fractions, complete resolution of all 12 components of the mixture was possible. In addition, a faster method was developed from online 2D HPLC analysis where multicomponent fractions from the first dimension are simultaneously chromatographed in the second dimension.
影响因子：
7.4
作者：
Filgueira, Marcelo R.;Huang, Yuan;Witt, Klaus;Castells, Cecilia;Carr, Peter W.
通讯作者：
Carr, Peter W.
影响因子：
7.4
作者：
Stoll, Dwight R.;Wang, Xiaoli;Carr, Peter W.
通讯作者：
Carr, Peter W.