Production of influenza virus proteins using recombinant insect cells
Production of influenza virus proteins using recombinant insect cells
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使用重组昆虫细胞生产流感病毒蛋白
DOI：
10.1051/matecconf/202133307009
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发表时间：
2020
影响因子：
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通讯作者：
Hideki Yamaji
中科院分区：
文献类型：
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作者：
Takuya Matsuda;Toshikazu Tanijima;Kyoko Masumi-Koizumi;Tomohisa Katsuda;Hideki Yamaji
流感疫苗长期以来一直在鸡胚中生产。这种方法存在一些问题，例如生产周期长（约6个月）和使用大量的传染性病原体。近年来，利用杆状病毒-昆虫细胞系统生产重组亚单位疫苗已被广泛研究。在该系统中，病毒免疫显性组分可以比常规的基于蛋的方法更快速地以更大的规模产生。然而，连续生产实际上是不可能的，因为重组杆状病毒的感染导致宿主昆虫细胞的死亡。在本研究中，我们建立了稳定转化的昆虫细胞，分泌流感病毒样颗粒（VLP）组成的血凝素（HA），流感病毒的主要保护性抗原，和基质蛋白1（M1），另一种结构蛋白的病毒。血凝试验和透射电镜（TEM）结果表明，重组昆虫细胞产生的HA具有血凝活性，且VLP的形态与野生型流感病毒颗粒相似。
Influenza vaccines have long been manufactured in embryonated chicken eggs. This method has some problems such as a long production period (about 6 months) and use of large amounts of infectious pathogens. Recently, the production of recombinant subunit vaccines using the baculovirus–insect cell system has been extensively investigated. In this system, viral immunodominant components can be produced more rapidly and in a larger scale than in the conventional egg-based process. However, continuous production is virtually impossible because infection of recombinant baculovirus results in the death of host insect cells. In the present study, we established stably transformed insect cells that secreted influenza virus-like particles (VLPs) consisting of hemagglutinin (HA), the major protective antigen of influenza A virus, and matrix protein 1 (M1), another structural protein of the virus. Hemagglutination assay and transmission electron microscopy (TEM) suggested that HA produced by recombinant insect cells kept the hemagglutination activity and the morphology of the VLPs was similar to that of wild type influenza virus particles.