L1 retrotransposons exploit RNA m(6)A modification as an evolutionary driving force.
L1 retrotransposons exploit RNA m(6)A modification as an evolutionary driving force.
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DOI：
10.1038/s41467-021-21197-1
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发表时间：
2021-02-09
影响因子：
16.6
通讯作者：
Ahn K
中科院分区：
文献类型：
--
作者：
Hwang SY;Jung H;Mun S;Lee S;Park K;Baek SC;Moon HC;Kim H;Kim B;Choi Y;Go YH;Tang W;Choi J;Choi JK;Cha HJ;Park HY;Liang P;Kim VN;Han K;Ahn K
L1 retrotransposons can pose a threat to genome integrity. The host has evolved to restrict L1 replication. However, mechanisms underlying L1 propagation out of the host surveillance remains unclear. Here, we propose an evolutionary survival strategy of L1, which exploits RNA m6A modification. We discover that m6A ‘writer’ METTL3 facilitates L1 retrotransposition, whereas m6A ‘eraser’ ALKBH5 suppresses it. The essential m6A cluster that is located on L1 5′ UTR serves as a docking site for eukaryotic initiation factor 3 (eIF3), enhances translational efficiency and promotes the formation of L1 ribonucleoprotein. Furthermore, through the comparative analysis of human- and primate-specific L1 lineages, we find that the most functional m6A motif-containing L1s have been positively selected and became a distinctive feature of evolutionarily young L1s. Thus, our findings demonstrate that L1 retrotransposons hijack the RNA m6A modification system for their successful replication. L1 is a group of active retrotransposons in humans. Here the authors show that m6A modifications on L1 RNA increase translation efficiency and retrotransposition in human cells. M6A motifs are more enriched in evolutionary young L1s.
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影响因子：
64.8
作者：
Jacobs, Frank M. J.;Greenberg, David;Ngan Nguyen;Haeussler, Maximilian;Ewing, Adam D.;Katzman, Sol;Paten, Benedict;Salama, Sofie R.;Haussler, David
通讯作者：
Haussler, David
影响因子：
5.3
作者：
Dmitriev, Sergey E.;Andreev, Dmitri E.;Shatsky, Ivan N.
通讯作者：
Shatsky, Ivan N.
影响因子：
3.5
作者：
Goodier, JL;Ostertag, EM;Kazazian, HH
通讯作者：
Kazazian, HH
DOI：
10.1073/pnas.0831042100
发表时间：
2003-04-29
影响因子：
11.1
作者：
Brouha, B;Schustak, J;Kazazian, HH
通讯作者：
Kazazian, HH
影响因子：
23.9
作者：
Batista, Pedro J.;Molinie, Benoit;Wang, Jinkai;Qu, Kun;Zhang, Jiajing;Li, Lingjie;Bouley, Donna M.;Lujan, Ernesto;Haddad, Bahareh;Daneshvar, Kaveh;Carter, Ava C.;Flynn, Ryan A.;Zhou, Chan;Lim, Kok-Seong;Dedon, Peter;Wernig, Marius;Mullen, Alan C.;Xing, Yi;Giallourakis, Cosmas C.;Chang, Howard Y.
通讯作者：
Chang, Howard Y.