Synthesis of UiO-66 loaded-caffeic acid and study of its antibacterial mechanism.
Synthesis of UiO-66 loaded-caffeic acid and study of its antibacterial mechanism.
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DOI：
10.1016/j.foodchem.2022.134248
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发表时间：
2022-09
期刊：
影响因子：
8.8
通讯作者：
Jianwei Zhou;Meimei Guo;Dan Wu;Mofei Shen;Donghong Liu;Tian Ding
中科院分区：
文献类型：
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作者：
Jianwei Zhou;Meimei Guo;Dan Wu;Mofei Shen;Donghong Liu;Tian Ding
锆基金属有机骨架（UiO-66）因其高载药量而成为天然化合物递送领域的重要材料。咖啡酸（CA）是一种物理化学稳定性不理想的天然化合物。CA@UiO-66提高CA的稳定性，抑制细菌的生长。通过水辅助法制备UiO-66对CA进行包封，CA@UiO-66对CA的负载率为56%，对CA的缓释率为83%，达到100h。大肠杆菌)和金黄色葡萄球菌(S。球菌)。最小杀菌浓度（MBC） forE。coliandS。金黄色葡萄球菌分别为1.0 mg/mL和2.0 mg/mL。3-（4,5-二甲基噻唑-2-基）2,5-二苯基溴化四唑（MTT）比色法表明，CA@UiO-66在MBC浓度下对人正常肝细胞LO2具有低毒或无毒作用。因此，CA@UiO-66在食品微生物安全领域具有潜力。
The Zirconium-based metal–organic framework (UiO-66) has become important in the field of natural compounds delivery for its high drug loading capacity. Caffeic acid (CA) is a natural compound with unsatisfactory physicochemical stability. CA@UiO-66 improved the stability of CA and inhibited the growth of bacteria. UiO-66 was prepared by water-assisted method for the encapsulation of CA. CA@UiO-66 showed the highest CA loading rate of 56 % and a sustained-release ability of CA of 83 % until 100 h. It destroyed the surface morphology and ultrastructure ofEscherichia coli(E. coli) andStaphylococcus aureus(S. aureus). The minimum bactericidal concentration (MBC) forE. coliandS. aureuswere 1.0 mg/mL and 2.0 mg/mL, respectively. The 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyl-tetrazolium bromide (MTT) colorimetry showed that the CA@UiO-66 had low toxicity or no toxicity to human normal liver cells LO2 at the concentration of MBC. Therefore, CA@UiO-66 has a potential in the field of food microbial safety.